Polynucleotides and polypeptides in plants
The invention relates to plant transcription factor polypeptides, polynucleotides that encode them, homologs from a variety of plant species, and methods of using the polynucleotides and polypeptides to produce transgenic plants having advantageous properties compared to a reference plant. Sequence information related to these polynucleotides and polypeptides can also be used in bioinformatic search methods and is also disclosed.
1. A method for producing a transgenic plant having greater yield, wherein the method comprises:
(a) providing a nucleic acid construct comprising a recombinant polynucleotide encoding a polypeptide that has a conserved domain that is at least 70% identical to amino acids 34-70 of SEQ ID NO: 38, wherein the polypeptide is at least 58% identical to the full length sequence of SEQ ID NO: 38;
(b) introducing the nucleic acid construct into a plant to produce a transgenic plant overexpressing said polypeptide;
(c) exposing the transgenic plant to a cold stress, a hyperosmotic stress, or a nitrogen-limited condition; and
(d) selecting a transgenic plant that has greater yield relative to a control plant not comprising said recombinant polynucleotide.
2. The method of claim 1 , wherein the polypeptide has a conserved domain that is at least 86% identical to amino acids 34-70 of SEQ ID NO: 38; and wherein the polypeptide is at least 78% identical to the full length sequence of SEQ ID NO: 38.
3. The method of claim 1 , the method steps further comprising:
(d) crossing the transgenic plant with itself or another plant; and
(e) selecting a transgenic seed that develops as a result of said crossing; and
(f) growing a progeny plant from the transgenic seed, thus producing a transgenic progeny plant having greater yield relative to the control plant, and wherein the progeny plant comprises said recombinant polynucleotide.
4. The method of claim 1 , wherein the polypeptide that has a conserved domain that is at least 83% identical to amino acids 34-70 of SEQ ID NO: 38; and wherein the polypeptide is at least 80% identical to the full length sequence of SEQ ID NO: 38.
5. A method for producing a transgenic plant having greater yield, wherein the method comprises the steps of:
(a) providing a nucleic acid construct comprising a recombinant polynucleotide encoding a polypeptide that is at least 90% identical to the full length sequence of SEQ ID NO: 38;
(b) introducing the nucleic acid construct into a plant to produce a transgenic plant overexpressing said polypeptide;
(c) exposing the transgenic plant to a cold stress, a hyperosmotic stress, or a nitrogen-limited condition; and
(d) selecting a transgenic plant that has greater yield relative to a control plant not comprising said recombinant polynucleotide.
6. A method for producing a transgenic plant having greater yield, wherein the method comprises the steps of:
(a) providing a nucleic acid construct comprising a recombinant polynucleotide encoding a polypeptide that has a conserved domain that is at least 70% identical to amino acids 34-70 of SEQ ID NO: 38, wherein the polypeptide is at least 58% identical to the full length sequence of SEQ ID NO: 38;
(b) introducing the nucleic acid construct into a plant to produce a transgenic plant overexpressing said polypeptide; and
(c) selecting a transgenic plant that has glabrous leaves, reduced trichome density, or increased number of root hairs relative to a control plant not comprising said recombinant polynucleotide, wherein the transgenic plant has greater yield relative to the control plant.
7. The method of claim 6 , wherein the polypeptide that has a conserved domain that is at least 83% identical to amino acids 34-70 of SEQ ID NO: 38; wherein the polypeptide is at least 80% identical to the full length sequence of SEQ ID NO: 38.
8. The method of claim 6 , wherein the polypeptide that has a conserved domain that is at least 86% identical to amino acids 34-70 of SEQ ID NO: 38, and wherein the polypeptide is at least 78% identical to the full length sequence of SEQ ID NO: 38.
9. The method of claim 8 , wherein the recombinant polynucleotide encodes a polypeptide that is at least 90% identical to the full length sequence of SEQ ID NO: 38.