Thermal and acid tolerant beta xylosidases, arabinofuranosidases, genes encoding, related organisms, and methods
Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof are provided. Further provided are methods of at least partially degrading xylotriose, xylobiose, and/or arabinofuranose-substituted xylan using isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius and variations thereof.
1. An isolated polypeptide comprising a polypeptide having at least 95% sequence identity to the polypeptide of SEQ ID NO:2;
wherein the polypeptide exhibits beta-xylosidase enzyme activity.
2. The isolated polypeptide of claim 1 , wherein the polypeptide exhibits enzymatic activity at or below about a pH of 8.
3. The isolated polypeptide of claim 1 , wherein the polypeptide exhibits enzymatic activity at a temperature at or above about 50degrees Celsius.
4. The isolated polypeptide of claim 1 , wherein the polypeptide is glycosylated, pegylated, or otherwise post-translationally modified.
5. The isolated polypeptide of claim 1 , wherein the polypeptide further exhibits arabinofuranosidase enzyme activity.
6. A method of at least partially degrading xylotriose or xylobiose, the method comprising:
providing the isolated polypeptide of claim 1 and placing the polypeptide in fluid contact with xylotriose or xylobiose.
7. A method of at least partially degrading arabinofuranose-substituted xylan, the method comprising:
providing the isolated polypeptide of claim 1 and placing the polypeptide in fluid contact with arabinofuranose-substituted xylan.
8. The method according to claim 7 , wherein the polypeptide exhibits arabinofuranosidase activity.
9. The isolated polypeptide of claim 5 , wherein the polypeptide exhibits enzymatic activity at or below about a pH of 8.
10. The isolated polypeptide of claim 5 , wherein the polypeptide exhibits enzymatic activity at a temperature at or above about 50 degrees Celsius.
11. The isolated polypeptide of claim 5 , wherein the polypeptide is glycosylated, pegylated, or otherwise post-translationally modified.