Crystalline anti-hTNFalpha antibodies

1. A crystal of an IgG human anti-hTNFalpha antibody comprising two heavy chains each with a molecular weight of about 50 kDa and comprising a light chain variable region (LCVR) comprising the amino acid sequence of SEQ ID NO: 1, and a heavy chain variable region (HCVR) comprising the amino acid sequence of SEQ ID NO: 2, wherein said crystal has a needle morphology with a length of about 2-500 μm and an l/d ratio of about 3 to 30.

2. The crystal according to claim 1 , wherein said antibody is selected from the group consisting of: IgG1, IgG2, IgG3 and IgG4 antibodies.

3. The crystal according to claim 1 , wherein said antibody is an IgG1 antibody.

4. The crystal according to claim 1 , wherein the antibody is adalimumab (D2E7).

5. A pharmaceutical composition comprising: (a) crystals of an anti-hTNFalpha antibody according to claim 1 , and (b) at least one pharmaceutical excipient; wherein the composition is provided as a solid, semisolid or liquid formulation, each formulation containing said antibody in crystalline form.

6. A pharmaceutical composition comprising: (a) crystals of an anti-hTNFalpha antibody according to claim 1 , and (b) at least one pharmaceutical excipient, which embeds or encapsulates crystals of said antibody.

7. The composition according to claim 5 , wherein said composition has an antibody concentration greater than about 1 mg/ml.

8. The composition according to claim 5 , wherein said composition has an antibody concentration greater than about 200 mg/ml.

9. The composition according to claim 5 , wherein said excipient comprises at least one polymeric optionally biodegradable carrier or at least one oil or lipid carrier.

10. The composition according to claim 9 , wherein said polymeric carrier is a polymer selected from one or more of the group consisting of: poly (acrylic acid), poly (cyanoacrylates), poly (amino acids), poly (anhydrides), poly (depsipeptide), poly (esters), poly (lactic acid), poly (lactic-co-glycolic acid) or PLGA, poly (β-hydroxybutryate), poly (caprolactone), poly (dioxanone); poly (ethylene glycol), poly (hydroxypropyl) methacrylamide, poly (organo) phosphazene, poly (ortho esters), poly (vinyl alcohol), poly (vinylpyrrolidone), maleic anhydride alkyl vinyl ether copolymers, pluronic polyols, albumin, alginate, cellulose and cellulose derivatives, collagen, fibrin, gelatin, hyaluronic acid, oligosaccharides, glycaminoglycans, sulfated polysaccharides, blends and copolymers thereof.

11. An injectable liquid composition comprising anti-hTNFalpha antibody crystals according to claim 1 and having an antibody concentration in the range of about 10 to 400 mg/ml.

12. A crystal slurry comprising anti-hTNFalpha antibody crystals according to claim 1 , having an antibody concentration greater than about 100 mg/ml.

13. A batch crystallization method for crystallizing an anti-hTNFalpha antibody, comprising the steps of:

(a) providing an aqueous solution of said antibody in admixture with an inorganic phosphate salt as a crystallization agent to obtain an aqueous crystallization mixture, wherein the aqueous crystallization mixture has a pH of about 3 to about 5; and

(b) incubating said aqueous crystallization mixture until crystals of said antibody are formed,

wherein the anti-hTNFalpha antibody is an IgG human anti-hTNFalpha antibody comprising a light chain variable region (LCVR) comprising the amino acid sequence of SEQ ID NO: 1, and a heavy chain variable region (HCVR) comprising the amino acid sequence of SEQ ID NO: 2, and wherein said crystal has a needle morphology with a length of about 2-500 μm and an l/d ratio of about 3 to 30.

14. The crystallization method according to claim 13 , wherein said aqueous crystallization mixture contains a buffer.

15. The crystallization method according to claim 14 , wherein said buffer comprises an acetate buffer.

16. The crystallization method according to claim 15 , wherein the buffer comprises sodium acetate.

17. The crystallization method according to claim 14 , wherein the buffer concentration in said aqueous crystallization mixture is about 0 M to about 0.5 M.

18. The crystallization method according to claim 15 , wherein the buffer concentration in said aqueous crystallization mixture is about 0 M to about 0.5 M.

19. The crystallization method according to claim 16 , wherein the buffer concentration in said aqueous crystallization mixture is about 0 M to about 0.5 M.

20. The crystallization method according to claim 13 , wherein the phosphate salt is a hydrogenphosphate salt.

21. The crystallization method according to claim 13 , wherein the phosphate salt is an alkali metal salt, or a mixture of at least two different alkali metal salts.

22. The crystallization method according to claim 13 , wherein the phosphate salt concentration in the crystallization mixture is in the range of about 1 to 6 M.

23. The crystallization method according to claim 22 , wherein the salt concentration in the crystallization mixture is in the range of about 1.0 to 3.0 M.

24. The crystallization method according to claim 13 , wherein at least one of the following additional crystallization conditions are met:

a) incubation is performed for between about 1 hour to about 60 days;

b) incubation is performed at a temperature between about 4° C. and about 37° C;

c) the antibody concentration is in the range of about 0.5 to about 100 mg/ml.

25. The crystallization method according to claim 24 , further comprising the step of drying said crystals.

26. The crystallization method according to claim 24 , further comprising the step of exchanging a crystallization mother liquor with a different buffer.

27. The crystallization method according to claim 24 , wherein the batch comprises a batch volume in the range of about 1 ml to about 20,000 liters.