Corn plant event MON87460 and compositions and methods for detection thereof
The present invention provides a transgenic corn even MON87460, and cells, seeds, and plants comprising DNA diagnostic for the corn event. The invention also provides compositions comprising nucleotide sequences that are diagnostic for MON87460 in a sample, methods for detecting the presence of MON87460 event polynucliotides in a sample, and probes and primers for use in detecting nucleotide sequences that are diagnostic for the presence of MON87460 in a sample. The present invention also provides methods of breeding with MON87460 to produce water deficit tolerance corn plants.
1. A corn plant having a chromosome comprising a transgene/genomic junction comprising the polynucleotide of SEQ ID NO:1 and a heterologous transgenic insert comprising a truncated rice actin promoter that is operably linked to a cspB gene, wherein the 5′ terminus of said insert overlaps the 3′ terminus of SEQ ID NO:1.
2. The corn plant of claim 1 , wherein said chromosome further comprises SEQ ID NO:2.
3. The corn plant of claim 2 , wherein said chromosome comprises SEQ ID NO:3, SEQ ID NO:4, SEQ ID NO:7 or SEQ ID NO:24.
4. The corn plant of claim 2 further comprising DNA for expressing a glyphosate resistant EPSPS.
5. A corn seed having a chromosome comprising a transgene/genomic junction comprising the polynucleotide of SEQ ID NO:1 and a heterologous transgenic insert comprising a truncated rice actin promoter that is operably linked to a cspB gene, wherein the 5′ terminus of said insert overlaps the 3′ terminus of SEQ ID NO:1.
6. The corn seed of claim 5 , wherein said chromosome further comprises SEQ ID NO:2.
7. The corn seed of claim 6 , wherein said chromosome comprises SEQ ID NO:7 or SEQ ID NO:24.
8. A processed food or feed commodity prepared from a corn seed having a chromosome comprising a transgene/genomic junction comprising the polynucleotide of SEQ ID NO:1 and a heterologous transgenic insert comprising a truncated rice actin promoter that is operably linked to a cspB gene, wherein the 5′ terminus of said insert overlaps a 3′ terminus of SEQ ID NO:1, and wherein said processed food or feed commodity comprises a detectable amount of a polynucleotide comprising a nucleotide sequence of SEQ ID NO: 1, SEQ ID NO: 2, or the complements thereof.
9. The processed food or feed commodity of claim 8 , wherein said food or said feed commodity comprises corn meal, corn flour, corn gluten, corn oil, or corn starch.
10. The processed food or feed commodity of claim 8 , wherein said polynucleotide comprises a nucleotide sequence of SEQ ID NO: 3, SEQ ID NO: 4, or the complements thereof.
11. The processed food or feed commodity of claim 8 , wherein said polynucleotide further comprises the nucleotide sequence of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, or SEQ ID NO:24.
12. A seed derived from a transgenic corn plant designated MON87460 or derived from progeny thereof, representative seed of said corn plant having been deposited under ATCC Accession No. PTA-8910, wherein said seed derived from a transgenic corn plant or derived from progeny thereof comprises SEQ ID NO:1.
13. The seed of claim 12 , wherein said seed has a chromosome comprising SEQ ID NO:1, SEQ ID NO:2, SEQ ID NO: 3, SEQ ID NO: 4, SEQ ID NO:7, or SEQ ID NO:24.
14. A method of detecting the presence of SEQ ID NO:1 or SEQ ID NO:2 in a corn tissue sample, the method comprising:
contacting the sample with a DNA primer pair, wherein said primer pair comprises: i) a first primer derived from the DNA sequence in the genome of the MON87460 plant adjacent to the insertion site of the inserted heterologous DNA (transgene DNA) and a second primer derived from the inserted heterologous DNA; or ii) two primers derived from genomic sequence on both sides of the inserted heterologous DNA; and
performing a polynucleic acid amplification reaction, thereby producing an amplicon; and
detecting said amplicon, wherein said amplicon comprises SEQ ID NO:1 and/or SEQ ID NO:2.
15. A polynucleotide comprising SEQ ID NO:2, or the complement thereof.
16. The polynucleotide of claim 15 , wherein said polynucleotide further comprises the nucleotide sequence of SEQ ID NO:4, SEQ ID NO: 6, or SEQ ID NO:7.
17. The method of claim 14 , wherein at least one DNA primer molecule in said primer pair comprises at least 11 consecutive nucleotides of SEQ ID NO:5, SEQ ID NO:6, SEQ ID NO:7, or the complements thereof.
18. The corn plant of claim 1 , wherein said heterologous transgenic insert confers water deficit tolerance.