Methods and compositions for imaging atherosclerotic plaques
The present invention relates in general to compositions, processes and apparatus for imaging, and in particular for preparation, collection and processing of images of atherosclerotic plaques, including images obtained from X-ray microscopic computed tomography.
1. A method for detecting an atherosclerotic plaque, said method comprising:
(a) incubating a specimen in a staining composition to produce a stained specimen, wherein said specimen comprises an aorta, heart and at least a portion of spine as an intact dissected sample, and wherein said staining composition comprises a 5% phosphotungstic acid (PTA) solution; and
(b) scanning said stained specimen in an X-ray tomography scanner to produce a microCT image of said stained specimen,
thereby detecting said atherosclerotic plaque.
2. The method of claim 1 , wherein prior to said incubating step (a), said specimen is fixed in a 10% neutral buffered formalin solution.
3. The method of claim 1 , wherein prior to said incubating step (a), said specimen is washed in a phosphate buffered saline (PBS) solution.
4. The method of claim 1 , wherein said incubating is conducted for at least two days.