Simian adenoviruses SAdV-36, -42.1, -42.2, and -44 and uses thereof

1. A recombinant adenovirus having a capsid comprising:

(a) a hexon protein of Simian Adenovirus 42.1 (SAdV-42.1), amino acids 1 to 957 of SEQ ID NO:43; a penton protein of SAdV-42.1, amino acids 1 to 581 of SEQ ID NO:38; and a fiber protein of SAdV-42.1, amino acids 1 to 577 of SEQ ID NO:52;

(b) a hexon protein of SAdV-42.2, amino acids 1 to 957 of SEQ ID NO:74; a penton protein of SAdV-42.2, amino acids 1 to 586 of SEQ ID NO:69; and a fiber protein of SAdV-42.2, amino acids 1 to 595 of SEQ ID NO:83; or

(c) a hexon protein of SAdV-44, amino acids 1 to 962 of SEQ ID NO:105; a penton protein of SAdV-44, amino acids 1 to 581 of SEQ ID NO:100; and a fiber protein of SAdV-44, amino acids 1 to 560 of SEQ ID NO:121;

said capsid encapsidating a heterologous nucleic acid molecule carrying a gene sequence operably linked to expression control sequences which direct transcription, translation, or expression in a host cell.

2. The adenovirus according to claim 1 , further comprising 5′ and 3′ adenovirus cis-elements necessary for replication and encapsidation.

3. The adenovirus according to claim 1 , wherein said adenovirus lacks all or a part of the E1 gene.

4. The adenovirus according to claim 3 , wherein said adenovirus is replication-defective.

5. The recombinant adenovirus according to claim 1 , wherein said adenovirus is a pseudotyped adenovirus comprising 5′ and 3′ adenovirus cis-elements necessary for replication and encapsidation, said cis-elements comprising an adenovirus 5′ inverted terminal repeat and an adenovirus 3′ inverted terminal repeat.

6. The recombinant adenovirus according to claim 1 , wherein the adenovirus comprises a nucleic acid sequence encoding a product operatively linked to sequences which direct expression of said product in a host cell.

7. The recombinant adenovirus according to claim 1 , wherein the recombinant adenovirus comprises one or more adenovirus genes.

8. The recombinant adenovirus according to claim 1 , wherein the recombinant adenovirus is replication-defective.

9. The recombinant adenovirus according to claim 8 , wherein the recombinant adenovirus is deleted in adenovirus E1.

10. A composition comprising a recombinant adenovirus according to claim 1 comprising the hexon, penton, and fiber capsid proteins of SAdV-42.1, wherein said recombinant adenovirus further comprises one or more simian adenovirus proteins selected from the group consisting of:

E1a, SEQ ID NO: 61;

E1b, small T/19K, SEQ ID NO: 54;

E1b, large T/55K, SEQ ID NO: 34;

IX, SEQ ID NO: 35;

52/55D, SEQ ID NO: 36;

IIIa, SEQ ID NO: 37;

VII, SEQ ID NO: 39;

V, SEQ ID NO: 40;

pX, SEQ ID NO: 41;

VI, SEQ ID NO: 42;

Endoprotease, SEQ ID NO: 44;

100 kD, SEQ ID NO: 45;

33 kD, SEQ ID NO: 63;

22 kD, SEQ ID NO: 56;

VIII, SEQ ID NO: 46;

12.5 K, SEQ ID NO: 57;

CR1-alpha, SEQ ID NO: 47;

gp19K, SEQ ID NO: 48;

CR1-beta, SEQ ID NO: 49;

CR1-gamma, SEQ ID NO: 58;

RID-alpha, SEQ ID NO: 50;

RID-beta, SEQ ID NO: 51; and

E3/14.7K, SEQ ID NO: 59; and 121.

11. A method for targeting a cell having an adenoviral receptor comprising delivering to a subject a composition according to claim 10 .

12. A composition comprising a recombinant adenovirus according to claim 1 in a pharmaceutically acceptable carrier.

13. A method for targeting a cell having an adenoviral receptor comprising delivering to a subject a recombinant adenovirus according to claim 1 .

14. A composition comprising a recombinant adenovirus according to claim 1 comprising the hexon, penton, and fiber capsid proteins of SAdV-42.2, wherein said recombinant adenovirus further comprises one or more simian adenovirus proteins selected from the group consisting of:

E1a, SEQ ID NO: 92;

E1b, small T/19K, SEQ ID NO: 65;

E1b, large T/55K, SEQ ID NO: 85;

IX, SEQ ID NO: 66;

52/55D, SEQ ID NO: 67;

IIIa, SEQ ID NO: 68;

VII, SEQ ID NO: 70;

V, SEQ ID NO: 71;

pX, SEQ ID NO: 72;

VI, SEQ ID NO: 73;

Endoprotease, SEQ ID NO: 75;

100 kD, SEQ ID NO: 76;

33 kD, SEQ ID NO: 94;

22 kD, SEQ ID NO: 87;

VIII, SEQ ID NO: 77;

12.5 K, SEQ ID NO: 78;

CR1-alpha, SEQ ID NO: 88;

gp19K, SEQ ID NO: 79;

CR1-beta, SEQ ID NO: 80;

CR1-gamma, SEQ ID NO: 89;

RID-alpha, SEQ ID NO: 81;

RID-beta, SEQ ID NO: 82; and

E3/14.7K, SEQ ID NO: 90.

15. A method for targeting a cell having an adenoviral receptor comprising delivering to a subject a composition according to claim 14 .

16. A composition comprising a recombinant adenovirus according to claim 1 comprising the hexon, penton, and fiber capsid proteins of SAdV-44, wherein said recombinant adenovirus further comprises one or more simian adenovirus proteins selected from the group consisting of:

E1a, SEQ ID NO: 123;

E1b, small T/19K, SEQ ID NO: 96;

E1b, large T/55K, SEQ ID NO: 116;

IX, SEQ ID NO: 97;

52/55D, SEQ ID NO: 98;

IIIa, SEQ ID NO: 99;

VII, SEQ ID NO: 101;

V, SEQ ID NO: 102;

pX, SEQ ID NO: 103;

VI, SEQ ID NO: 104;

Endoprotease, SEQ ID NO: 106;

100 kD, SEQ ID NO: 107;

33 kD, SEQ ID NO: 125;

22 kD, SEQ ID NO: 118;

VIII, SEQ ID NO: 108;

12.5 K, SEQ ID NO: 109;

CR1-alpha, SEQ ID NO: 110;

gp19K, SEQ ID NO: 111;

CR1-beta, SEQ ID NO: 112;

CR1-gamma, SEQ ID NO: 119;

RID-alpha, SEQ ID NO: 113;

RID-beta, SEQ ID NO: 114; and

E3/14.7K, SEQ ID NO: 120.

17. A method for targeting a cell having an adenoviral receptor comprising delivering to a subject a composition according to claim 16 .