Anti-ILT7 antibody
An antibody binding to IPC was obtained by using an animal cell in which a cell membrane protein associatable with ILT7 was co-expressed as an immunogen. The antibody of the invention has a high specificity which allows immunological distinction between other ILT family molecules and ILT7. The anti-ILT7 antibody of the invention bound to IPC and inhibited the activity thereof. With the anti-ILT7 antibody of the invention, the IPC activity can be inhibited and an interferon-related disease can be treated or prevented. ILT7 expression is maintained even in IPC in the presence of IFNα. Therefore, an inhibitory action of IPC activity by the anti-ILT7 antibody can be expected even in an autoimmune disease patient with an increased production of IFNα.
1. An isolated polynucleotide encoding a monoclonal antibody which binds to an extracellular domain of human ILT7 or a fragment comprising its antigen binding region wherein the monoclonal antibody comprises an amino acid sequence selected from any of the following combinations (a) to (c) as the heavy chain variable region and the light chain variable region:
a) a heavy chain variable region of SEQ ID NO: 39 and a light chain variable region of SEQ ID NO: 41;
b) a heavy chain variable region of SEQ ID NO: 43 and a light chain variable region of SEQ ID NO: 45; and
c) a heavy chain variable region of SEQ ID NO: 47 and a light chain variable region of SEQ ID NO: 49.
2. A vector comprising the polynucleotide according to claim 1 .
3. An isolated transformed cell retaining the vector according to claim 2 in an expressible manner.
4. A method for producing a monoclonal antibody which binds to an extracellular domain of human ILT7, comprising the steps of: culturing the transformed cell according to claim 3 ; and recovering the monoclonal antibody from the culture.