Cytotoxic T-cell epitope peptides that specifically attack epstein-barr virus-infected cells and uses thereof
The present inventors introduced mRNAs for the Epstein-Barr virus proteins LMP1 and EBNA1 into antigen-presenting cells, and as a result, demonstrated that the cells induced Epstein-Barr virus-specific cytotoxic T cells. The present inventors also demonstrated that the cytotoxic T cells recognized epitope peptides presented via HLA-A*0206, HLA-Cw*0303, or HLA-Cw*0304, inhibited the outgrowth of Epstein-Barr virus-infected B cells, and lysed Epstein-Barr virus-infected NK lymphomas and NK cells.
1. An isolated Epstein-Barr virus-specific cytotoxic T cell epitope peptide, which binds an HLA Class I molecule and which consists of an amino acid sequence as shown in SEQ ID NO: 1.
2. A vaccine for treating or preventing Epstein-Barr virus infection, which comprises as an active ingredient the peptide of claim 1 .
3. A method for inducing an Epstein-Barr virus-specific cytotoxic T cell response, which comprises contacting a cytotoxic T cell with the peptide of claim 1 .
4. The method of claim 3 , further comprising contacting the peptide of claim 1 with a peripheral blood mononuclear cell in a culture medium containing plasma.