Device for studying individual cells
A device for studying individual cells including a picowell array (such as an array of microwells, dimples, depressions, tubes or enclosures) and a fluid reservoir in fluid communication with the picowells through channels is disclosed. Preferably, the device has a moveable lid that in one rest location allows loading of cells in the picowell array. Preferably the channels of the device are capillary channels. Also disclosed is a device for the automated study of cells including a picowell-bearing device having a picowell array with a moveable lid, a lid-moving component, at least one solution dispensing component and a control system functionally associated with the lid-moving component as well as with the solution dispensing component.
1. A method of using a picowell-bearing device comprising: loading a solution comprising cells directly into at least some of the wells of a picowell array; placing a lid above said array; and delivering a fluid through a capillary channel in fluid communication with said picowell array, said capillary channel configured to draw a fluid towards said picowell array using only capillary force and without the use of flow generators.
2. The method of claim 1 , further comprising adding a reagent to a fluid reservoir, in fluid communication with said capillary channel, wherein said reagent is comprised in said fluid delivered through said capillary channel.
3. The method of claim 1 , further comprises opening said lid to remove a wall of said capillary channel, wherein a flow of said fluid stops in said open capillary channel.
4. The method of claim 1 , wherein said lid is placed at a distance above said picowell array, said distance selected to prevent cells from being carried away by said delivered fluid.
5. The method of claim 1 , further comprising regulating a flow rate of fluid in said capillary channel.
6. The method of claim 5 , wherein said flow rate is regulated by changing a dimension of said capillary channel.
7. The method of claim 1 , wherein placing said lid seals said picowell array and said capillary channel.
8. The method of claim 1 , further comprising regulating a flow of said fluid away from said picowell array towards a waste reservoir through a capillary waste channel.
9. The method of claim 8 , wherein regulating comprises regulating a volume of said waste reservoir.
10. The method according to claim 1 , and further comprising: staining said cells by delivering a staining fluid to said array through said capillary channel.
11. The method of claim 10 , wherein said staining fluid is delivered to said picowell array from a fluid reservoir in fluid communication with said capillary channel.
12. The method of claim 10 , wherein placing said lid over said picowell array forms one wall of said capillary channel.
13. The method of claim 12 , wherein the lid when placed over said picowell array is located at a distance above said picowell array, said distance selected to maintain the cells in a stable environment.
14. The method of claim 12 , further including regulating fluid flow through said capillary channel by changing a position of said lid.
15. The method of claim 1 , further comprising providing a waste reservoir at a downstream end of said capillary channel.
16. The method of claim 1 , further comprising washing said cells by delivering a washing fluid to said array through said capillary channel.
17. The method of claim 16 , wherein said washing fluid is delivered to said array from a fluid reservoir through said capillary channel.