Optical nanosensors comprising photoluminescent nanostructures
Systems and methods related to optical nanosensors comprising photoluminescent nanostructures are generally described. Generally, the nanosensors comprise a photoluminescent nanostructure and a polymer that interacts with the photoluminescent nanostructure. In some cases, the interaction between the polymer and the nanostructure can be non-covalent (e.g., via van der Waals interactions). The nanosensors comprising a polymer and a photoluminescent nanostructure may be particularly useful in determining the presence and/or concentration of relatively small molecules, in some embodiments. In addition, in some instances the nanosensors may be capable of determining relatively low concentrations of analytes, in some cases determining as little as a single molecule. In some embodiments, the interaction between the analyte and the nanosensor (e.g., between the analyte and the photoluminescent nanostructure) can be reversible, which may allow, for example, for the reuse of a nanosensor after it has been exposed to an analyte.
1. A nanosensor for detecting an analyte, comprising:
a photoluminescent nanostructure; and
a polymer including a polysaccharide modified to enhance the detection of the analyte interacting with the photoluminescent nanostructure, wherein
the nanosensor emits a first emission of electromagnetic radiation in the absence of the analyte, and
the nanosensor emits a second emission of electromagnetic radiation upon interacting with the analyte at concentrations of less than about 100 micromolar.
2. The nanosensor of claim 1 , wherein the analyte has a molecular weight of less than 100 g/mol.
3. The nanosensor of claim 2 , wherein the analyte is nitric oxide.
4. The nanosensor of claim 1 , wherein the photoluminescent nanostructure comprises a carbon nanotube.
5. The nanosensor of claim 4 , wherein the carbon nanotube is a single-walled carbon nanotube.
6. The nanosensor of claim 5 , wherein the single-walled carbon nanotube is a semiconductive single-walled carbon nanotube.
7. The nanosensor of claim 1 , wherein the photoluminescent nanostructure emits near-infrared radiation in the absence of the analyte.
8. The nanosensor of claim 1 , wherein the photoluminescent nanostructure emits near-infrared radiation in the presence of the analyte.
9. The nanosensor of claim 1 , wherein the nanosensor emits electromagnetic radiation of a first intensity in the absence of the analyte, and the nanosensor emits electromagnetic radiation of a second intensity that is less than the first intensity upon interacting with the analyte.
10. The nanosensor of claim 1 , wherein the analyte is analyte within a cell.
11. The nanosensor of claim 1 , wherein the analyte is produced by a cell.
12. The nanosensor of claim 1 , wherein the concentration of the analyte is less than 1 micromolar.
13. The nanosensor of claim 1 , wherein the interaction between the analyte and the nanosensor includes an interaction between the analyte and the photoluminescent nanostructure.
14. A method, comprising:
exposing a nanosensor comprising a photoluminescent nanostructure and a polymer including a polysaccharide modified to enhance the detection of the analyte interacting with the photoluminescent nanostructure, wherein
the nanosensor emits a first emission of electromagnetic radiation in the absence of the analyte, and
the nanosensor emits a second emission of electromagnetic radiation upon interacting with the analyte at concentrations of less than about 100 micromolar, to a solution containing an analyte at a concentration of less than about 100 micromolar; and
determining the analyte based upon an interaction between the analyte and the nanosensor.
15. The method of claim 14 , wherein the analyte has a molecular weight of less than 100 g/mol.
16. The method of claim 15 , wherein the analyte is nitric oxide.
17. The method of claim 14 , wherein the photoluminescent nanostructure comprises a carbon nanotube.
18. The method of claim 17 , wherein the carbon nanotube is a single-walled carbon nanotube.
19. The method of claim 18 , wherein the single-walled carbon nanotube is a semiconductive single-walled carbon nanotube.
20. The method of claim 14 , further comprising exposing the photoluminescent nanostructure to the interior of a cell.
21. The method of claim 14 , further comprising determining an analyte within a cell.
22. The method of claim 14 , wherein the photoluminescent nanostructure emits near-infrared radiation in the absence of the analyte.
23. The method of claim 14 , wherein the photoluminescent nanostructure emits near-infrared radiation in the presence of the analyte.
24. The method of claim 14 , wherein the nanosensor emits electromagnetic radiation of a first intensity in the absence of the analyte, and the nanosensor emits electromagnetic radiation of a second intensity that is less than the first intensity upon interacting with the analyte.
25. The method of claim 14 , further comprising determining an analyte produced by a cell.
26. The method of claim 14 , further comprising determining an analyte for the diagnosis or management of at least one of asthma, rheumatoid arthritis, multiple sclerosis, tuberculosis, Alzheimer's disease, and cancer.
27. The method of claim 14 , wherein the concentration of the analyte is less than 1 micromolar.
28. The method of claim 14 , wherein the interaction between the analyte and the nanosensor comprises an interaction between the analyte and the photoluminescent nanostructure.
29. The method of claim 14 , further comprising determining the presence of a single analyte molecule.