Modified 13-hydroperoxide lyases and uses thereof
Fatty acid 13-hydroperoxide lyase proteins which have been modified with respect to a previously described guava 13-hydroperoxide lyase and the nucleic acid sequences encoding these proteins. Also, recombinant nucleic acid molecules for expressing the modified 13-hydroperoxide lyases and methods of using such lyases in the field of organic synthesis.
1. A modified 13-hydroperoxide lyase having an amino acid sequence selected from the group consisting of SEQ ID Nos. 2, 4, 6, 8, 10, 12, 14 or 16, wherein said modified 13-hydroperoxide lyase has 13-hydroperoxide lyase activity.
2. A method for preparing a modified 13-hydroperoxide lyase polypeptide which comprises:
culturing an isolated host cell transformed with a nucleotide sequence encoding the modified 13-hydroperoxide lyase of claim 1 under conditions which permit expression of the modified 13-hydroperoxide lyase polypeptide, and, optionally
recovering the modified 13-hydroperoxide lyase polypeptide.