Fluorescent probe
A compound represented by the following general formula (I): [wherein R 1 and R 2 represent a C 1-6 alkyl group; R 3 , R 4 and R 5 represent hydrogen atom, a C 1-6 alkyl group, or an aryl group; Y 1 and Y 2 represent —O—, —S—, —Se—, —CH═CH—, —C(R 6 )(R 7 )—, or —N(R 8 )— (wherein R 6 , R 7 and R 8 represent hydrogen atom or a C 1-6 alkyl group); A represents a C 1-3 alkylene group; n and n′ represent 0, 1 or 2; Z 1 and Z 2 represent a nonmetallic atom group required to form a benzo-condensed ring or a naphtho-condensed ring; L 1 to L 7 represent a methine group; and M − represents a counter ion in a number required for neutralizing electrical charge], which is useful as a fluorescent probe of which optical characteristics change depending on pH change.
1. A compound having one of the following structures:
wherein M − represents a counterion in a number required for neutralizing electrical charge.
2. A compound according to claim 1 having the following structure:
3. A pH fluorescent probe containing the compound according to claim 2 .
4. A method for measuring pH of a test object, which comprises:
(a) contacting the compound of claim 2 with the test object, and
(b) measuring fluorescence intensity of the resulting compound after the contacting in (a).
5. The method according to claim 4 , wherein the measuring fluorescence intensity of (b) includes excitation with two different wavelengths and measuring a ratio of fluorescent intensities.
6. The method according to claim 5 , wherein the test object is a cell or a tissue.
7. A compound according to claim 1 having the following structure:
8. A pH fluorescent probe containing the compound according to claim 7 .
9. A method for measuring pH of a test object, which comprises:
(a) contacting the compound of claim 7 with the test object, and
(b) measuring fluorescence intensity of the resulting compound after the contacting in (a).
10. The method according to claim 9 , wherein the measuring fluorescence intensity of (b) includes excitation with two different wavelengths and measuring a ratio of fluorescent intensities.
11. The method according to claim 10 , wherein the test object is a cell or a tissue.
12. A compound according to claim 1 having the following structure:
13. A pH fluorescent probe containing the compound according to claim 12 .
14. A method for measuring pH of a test object, which comprises:
(a) contacting the compound according to claim 12 with the test object, and
(b) measuring fluorescence intensity of the resulting compound after the contacting in (a).
15. The method according to claim 14 , wherein the measuring fluorescence intensity of (b) includes excitation with two different wavelengths and measuring a ratio of fluorescent intensities.
16. The method according to claim 14 , wherein the test object is a cell or a tissue.
17. A compound according to claim 1 having the following structure:
18. A pH fluorescent probe containing the compound according to claim 17 .
19. A method for measuring pH of a test object, which comprises:
(a) contacting the compound according to claim 17 with the test object, and
(b) measuring fluorescence intensity of the resulting compound after the contacting in (a).
20. The method according to claim 19 , wherein the test object is a cell or a tissue.
21. The method according to claim 19 , wherein the measuring fluorescence intensity of (b) includes excitation with two different wavelengths and measuring a ratio of fluorescent intensities.
22. The method according to claim 21 , wherein the test object is a cell or a tissue.
23. A compound according to claim 1 having the following structure:
24. A pH fluorescent probe containing the compound according to claim 23 .
25. A method for measuring pH of a test object, which comprises:
(a) contacting the compound of claim 23 with the test object, and
(b) measuring fluorescence intensity of the resulting compound after the contacting in (a).