Collagen preparation and method of isolation
Collagen compositions, methods for preparing those collagen compositions, and graft compositions formed from those collagen compositions are provided. In particular, methods of isolating collagen that exhibits an enhanced rate of polymerization and enhanced microstructural and mechanical properties upon polymerization, such collagen compositions, and graft compositions formed from such collagen compositions are provided.
1. An engineered matrix comprising collagen oligomers wherein the collagen oligomers comprise β and γ chains of collagen, and wherein the matrix further comprises an intermediate collagen oligomer with a molecular weight between the β and γ chains of collagen, and wherein the collagen in the matrix is porcine skin collagen.
2. The matrix of claim 1 wherein the collagen oligomers are solubilized from a tissue using a citrate solution.
3. The matrix of claim 1 wherein the collagen in the matrix is crosslinked.
4. The matrix of claim 1 further comprising collagen monomers.
5. The matrix of claim 1 wherein the matrix is sterilized with peracetic acid.
6. The matrix of claim 1 wherein the matrix further comprises cells.
7. The matrix of claim 1 wherein the matrix further comprises a glycoprotein, a glycosaminoglycan, or a proteoglycan.
8. The matrix of claim 1 wherein the collagen used to form the matrix is not digested with an enzyme.
9. The matrix of claim 8 wherein the collagen used to form the matrix is not digested with pepsin.
10. A graft composition comprising an engineered matrix comprising collagen oligomers wherein the collagen oligomers comprise β and γ chains of collagen, and wherein the matrix further comprises an intermediate collagen oligomer with a molecular weight between the β and γ chains of collagen, and wherein the collagen in the matrix is porcine skin collagen.
11. The graft of claim 10 wherein the collagen oligomers are solubilized from a tissue using a citrate solution.
12. The graft of claim 10 wherein the collagen in the matrix is crosslinked.
13. The graft of claim 10 further comprising collagen monomers.
14. The graft of claim 10 wherein the graft is sterilized with peracetic acid.
15. The graft of claim 10 wherein the graft further comprises cells.
16. The graft of claim 10 wherein the graft further comprises a glycoprotein, a glycosaminoglycan, or a proteoglycan.
17. The graft of claim 10 wherein the graft is implantable.
18. The graft of claim 10 wherein the graft is injectable.
19. The graft of claim 10 wherein the collagen used to form the matrix is not digested with an enzyme.
20. The graft of claim 19 wherein the collagen used to form the matrix is not digested with pepsin.