Method for preserving liver in a solution comprising dibutyryl cAMP
The present invention provides a liver preservation solution containing trehalose and dibutyryl-cAMP. The content of nitroglycerin in the preservation solution is preferably lower than 0.44 mM. In the liver preservation solution of the present invention, since the toxicity due to nitroglycerin, which is observed during liver preservation, has been improved, liver transplantation can be performed with a high engrafted rate.
1. A method of preserving liver, which comprises preserving an isolated liver in a tissue preservation solution comprising at least the following components within the following ranges and having a nitroglycerin content of not more than 0.0044 mM:
dibutyryl cAMP
1.5-3
mM
trehalose
110-130
mM
hydroxyethylstarch
25-35
g/l
Na +
90-110
mM
K +
40-50
mM
H 2 PO 4 − or HPO 4 −−
20-30
mM
at least one kind selected from
90-110
mM.
Cl − , HCO 3 − , CO 3 −− , organic acid and
organic acid anion
2. The method of claim 1 , wherein the tissue preservation solution is substantially free of nitroglycerin.