IP Library › Granted Patent US 8,524,455
Granted Patent B2
US 8,524,455 · App. 12/842,569 · Granted Sep 3, 2013

Methods and compositions related to TR4 ligand

Inventor: Chawnshang Chang (Pittsford, NY)
Assignee: University of Rochester
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Quick Facts
Patent No.
US 8,524,455
App. No.
12/842,569
Granted
Sep 3, 2013
Kind
B2
Abstract

Disclosed are compositions and methods related to TR4 and aging.

Claims (41)

1. A method of isolating drug candidates that do not bind TR4 and bind PPAR comprising:

a. incubating drug candidates with TR4;

b. isolating drug candidates that do not bind TR4;

c. incubating the TR4 non-binding drug candidates with PPAR; and

d. isolating the TR4 non-binding drug candidates that bind PPAR.

2. The method of claim 1 wherein incubating the drug candidates with TR4, comprises combining drug candidates and TR4 in a mixture allowing for contact between the drug candidates and TR4.

3. The method of claim 1 wherein isolating drug candidates that do not bind TR4 comprises:

passing the drug candidates over a TR4 linked to a support.

4. The method of claim 3 wherein the support linked to TR4 comprises sepharose, an agarose bead or an antibody.

5. The method of claim 3 wherein the linked TR4 comprises a covalent bond between the support and TR4.

6. The method of claim 3 wherein the linked TR4 comprises a non-covalent bond between the support and TR4.

7. The method of claim 1 wherein isolating the drug candidates that bind PPAR comprises:

a. passing the TR4 non-binding drug candidates over PPAR linked to a support; and

b. eluting the drug candidates bound to the linked PPAR.

8. The method of claim 7 wherein the support linked to TR4 comprises sepharose, agarose beads or antibodies.

9. The method of claim 7 wherein the linked PPAR comprises a covalent bond between the support and PPAR.

10. The method of claim 7 wherein the linked PPAR comprises a non-covalent bond between the support and PPAR.

11. The method of claim 7 wherein eluting the drug candidates bound to linked PPAR comprises: washing the linked PPAR with a wash comprising an elution molecule; washing the linked PPAR with a wash comprising a low or high pH buffer; or washing the linked PPAR with a wash comprising a detergent.

12. The method of claim 11 wherein the elution molecule is a polyunsaturated fatty acid (PUFA).

13. The method of claim 12 , wherein the PUFA comprises an omega-3 fatty acid.

14. A method of isolating drug candidates that do not bind PPAR and bind TR4 comprising:

a. incubating drug candidates with PPAR;

b. isolating drug candidates that do not bind PPAR;

c. incubating the PPAR non-binding drug candidates with TR4; and

d. isolating the PPAR non-binding drug candidates that bind TR4.

15. The method of claim 14 wherein incubating the drug candidates with PPAR, comprises combining drug candidates and PPAR in a mixture allowing for contact between the drug candidates and PPAR.

16. The method of claim 14 wherein isolating drug candidates that do not bind PPAR comprises:

passing the drug candidates over PPAR linked to a support.

17. The method of claim 16 wherein the support linked to PPAR comprises sepharose, an agarose bead or an antibody.

18. The method of claim 16 wherein the linked PPAR comprises a covalent bond between the support and PPAR.

19. The method of claim 16 wherein the linked PPAR comprises a non-covalent bond between the support and PPAR.

20. The method of claim 14 wherein isolating the drug candidates that bind TR4 comprises:

a. passing the PPAR non-binding drug candidates over TR4 linked to a support; and

b. eluting the drug candidates bound to the linked TR4.

21. The method of claim 20 , wherein the support linked to PPAR comprises sepharose, agarose beads or antibodies.

22. The method of claim 20 , wherein the linked TR4 comprises a covalent bond between the support and TR4.

23. The method of claim 20 wherein the linked TR4 comprises a non-covalent bond between the support and TR4.

24. The method of claim 20 wherein eluting the drug candidates bound to linked TR4 comprises: washing the linked TR4 with a wash comprising an elution molecule; washing the linked TR4 with a wash comprising a low or high pH buffer; or washing the linked TR4 with a wash comprising a detergent.

25. The method of claim 24 wherein the elution molecule comprises an omega-3 fatty acid.

26. The method of claim 1 , wherein the drug candidates are drugs or small molecules.

27. The method of claim 14 , wherein the drug candidates are drugs or small molecules.

Assignments (3)
GOVERNMENT INTEREST AGREEMENT Recorded Apr 18, 2019
From: UNIVERSITY OF ROCHESTER
To: NATIONAL INSTITUTES OF HEALTH - DIRECTOR DEITR
Reel/Frame 048944/0060 →
CONFIRMATORY LICENSE Recorded Apr 27, 2012
From: UNIVERSITY OF ROCHESTER
To: NATIONAL INSTITUTES OF HEALTH (NIH), U.S. DEPT. OF HEALTH AND HUMAN SERVICES (DHHS), U.S. GOVERNMENT
Reel/Frame 028116/0789 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 15, 2010
From: CHANG, CHAWNSHANG
To: UNIVERSITY OF ROCHESTER
Reel/Frame 025144/0629 →
Continuity (3)
Provisional Application 61228537 · Jul 24, 2009
Provisional Application 61229223 · Jul 28, 2009
Related Publication 20110028538A1 · Feb 3, 2011