Fructanase
The present invention relates to a novel protein and a method for the manufacture thereof. The novel protein according to the invention is a recombinant protein with fructanase activity. The recombinant protein according to the invention is an engineered protein derived from recombinant DNA encoding for the protein. The recombinant protein may be or may comprise a fragment of a naturally occurring protein, i.e. of a naturally occurring fructanase protein.
1. An isolated protein with fructanase activity, wherein the protein comprises the amino acid sequence of SEQ ID NO:3.
2. The isolated protein according to claim 1 , wherein the protein is isolated from Lactobacillus paracasei.
3. The isolated protein according to claim 1 , wherein the protein further comprises at least one histidine residue.
4. The isolated protein according to claim 1 , wherein the protein comprises a tag.
5. The isolated protein according to claim 1 , wherein the fructanase activity comprises the ability to hydrolyse a fructan.
6. The isolated protein according to claim 5 , wherein the fructan is at least one of inulin, levan, 1-kestose, nystose, rafinose, stachyose and melezitose or a combination thereof.
7. The isolated protein according to claim 1 , wherein the protein is soluble in an aqueous solution.
8. The isolated protein according to claim 1 , recombinantly expressed in a prokaryotic or an eukaryotic cell.