Gel phantoms for testing cavitational ultrasound (histotripsy) transducers
A cavitational ultrasound (e.g., Histotripsy) gel phantom and cavitational ultrasound testing system are provided that may include any of a number of features. One feature of the phantom and system is that it can allow for instant visual feedback on the efficacy and dosage of a Histotripsy transducer. The changes in the gel phantom can be visualized with the naked eye without having to wait for histology. The changes in the gel phantom can also be visualized with a camera, with ultrasound imaging, or with microscopy. In various embodiments, the phantom includes indicators such as carbon particles, dye-encapsulated beads, and red blood cells. Methods associated with use of the cavitational ultrasound gel phantom and testing system are also covered.
1. A cavitational damage indicator phantom, comprising:
a container;
a gel disposed in the container; and
an indicator disposed in the container, the indicator configured to change visibly during application of cavitational ultrasound energy to the indicator.
2. The cavitational damage indicator phantom of claim 1 wherein the indicator is embedded between first and second layers of the gel.
3. The cavitational damage indicator phantom of claim 2 wherein the indicator comprises a layer having a thickness of less than 5 mm.
4. The cavitational damage indicator phantom of claim 1 wherein the gel comprises an agarose gel.
5. The cavitational damage indicator phantom of claim 1 wherein the indicator comprises a plurality of carbon particles.
6. The cavitational damage indicator phantom of claim 5 wherein the visible change comprises the carbon particles becoming darker during application of cavitational ultrasound energy to the indicator.
7. The cavitational damage indicator phantom of claim 5 wherein the visible change comprises a breakdown of the size of the carbon particles.
8. The cavitational damage indicator phantom of claim 1 wherein the indicator comprises a plurality of microspheres.
9. The cavitational damage indicator phantom of claim 8 wherein the microspheres comprise polystyrene beads.
10. The cavitational damage indicator phantom of claim 8 wherein the microspheres comprise microencapsulated beads.
11. The cavitational damage indicator phantom of claim 8 wherein the visible change comprises spilling a pigment from the microspheres during application of cavitational ultrasound energy to the indicator.
12. The cavitational damage indicator phantom of claim 1 wherein the indicator comprises red blood cells.
13. The cavitational damage indicator phantom of claim 1 wherein the visible change of the indicator can be detected by a human eye.
14. The cavitational damage indicator phantom of claim 1 wherein the visible change comprises a color change.
15. The cavitational damage indicator phantom of claim 6 wherein the indicator comprises a plurality of layers disposed in the container.
16. The cavitational damage indicator phantom of claim 1 wherein the container is at least partially transparent.
17. The cavitational damage indicator phantom of claim 1 wherein the gel is transparent.
18. The cavitational damage indicator phantom of claim 1 wherein the gel is translucent.
19. A cavitational damage testing system, comprising:
a tank filled with an acoustic medium; and
a cavitational ultrasound phantom disposed in the tank, the cavitational ultrasound phantom comprising a container, a gel disposed in the container, and an indicator disposed in the container, the indicator configured to change visibly under application of cavitational ultrasound energy to the indicator.
20. The cavitational damage testing system of claim 19 wherein the indicator is embedded between first and second layers of the gel.
21. The cavitational damage testing system of claim 20 wherein the indicator comprises a layer having a thickness <5 mm.
22. The cavitational damage testing system of claim 19 wherein the gel comprises a transparent agarose gel.
23. The cavitational damage testing system of claim 19 wherein the indicator comprises a plurality of carbon particles.
24. The cavitational damage testing system of claim 19 wherein the indicator comprises a plurality of microspheres.
25. The cavitational damage testing system of claim 24 wherein the microspheres comprise polystyrene beads.
26. The cavitational damage testing system of claim 24 wherein the microspheres comprise microencapsulated beads.
27. The cavitational damage testing system of claim 19 wherein the indicator comprises red blood cells.
28. The cavitational damage testing system of claim 19 further comprising a mount disposed on or near the tank, the mount configured to receive a cavitational ultrasound transducer.
29. The cavitational damage testing system of claim 28 wherein the mount is configured to align a focal point of the cavitational ultrasound transducer with the cavitational ultrasound phantom.
30. The cavitational damage testing system of claim 19 further comprising a cavitational ultrasound therapy transducer configured to deliver cavitational ultrasound energy to the cavitational ultrasound phantom.
31. The cavitational damage testing system of claim 19 further comprising an imaging system.
32. The cavitational damage testing system of claim 31 wherein the imaging system comprises a high-speed video camera.
33. The cavitational damage testing system of claim 19 wherein the acoustic medium comprises degassed water.
34. A method of testing a Histotripsy transducer, comprising:
applying Histotripsy energy to a Histotripsy phantom; and
observing a visual change in the Histotripsy phantom.
35. The method of claim 34 wherein the visual change comprises a color change.
36. The method of claim 34 wherein the Histotripsy phantom comprises carbon particles disposed in a transparent gel and the visual change comprises observing the carbon particles change to a darker color.
37. The method of claim 34 wherein the Histotripsy phantom comprises microspheres disposed in a transparent gel and the visual change comprises releasing a dye from the microspheres during application of Histotripsy energy to the Histotripsy phantom.
38. The method of claim 34 further comprising, prior to the applying step:
placing the Histotripsy phantom in a testing tank; and
aligning a focal point of a Histotripsy transducer with the Histotripsy phantom.
39. The method of claim 38 wherein the applying Histotripsy energy step comprises applying Histotripsy energy to the Histotripsy phantom with the Histotripsy transducer.
40. The method of claim 34 wherein the Histotripsy phantom resembles a human prostate.
41. The method of claim 34 wherein the visual change observed in the Histotripsy phantom is indicative of tissue damage to be expected during a Histotripsy procedure in a human patient.