IP Library › Granted Patent US 8,569,030
Granted Patent B2
US 8,569,030 · App. 13/604,979 · Granted Oct 29, 2013

Type II restriction-modification system methylation subunit of

Inventors: Brady D. Lee (Idaho Falls, ID); Deborah T. Newby (Idaho Falls, ID); Jeffrey A. Lacey (Idaho Falls, ID); David N. Thompson (Idaho Falls, ID); Vicki S. Thompson (Idaho Falls, ID); William A. Apel (Jackson, WY); Francisco F. Roberto (Idaho Falls, ID); David W. Reed (Idaho Falls, ID)
Assignee: Battelle Energy Alliance, LLC
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Quick Facts
Patent No.
US 8,569,030
App. No.
13/604,979
Granted
Oct 29, 2013
Kind
B2
Abstract

Isolated and/or purified polypeptides and nucleic acid sequences encoding polypeptides from Alicyclobacillus acidocaldarius are provided. Further provided are methods for modulating or altering recombination inside or outside of a cell using isolated and/or purified polypeptides and/or nucleic acid sequences from Alicyclobacillus acidocaldarius.

Claims (7)

1. An isolated nucleic acid sequence comprising a nucleic acid sequence encoding a polypeptide having at least 90% sequence identity to SEQ ID NO: 2976.

2. The isolated nucleic acid sequence of claim 1 , wherein the polypeptide has activity at or below about pH 8.

3. The isolated nucleic acid sequence of claim 1 , wherein the polypeptide has activity at a temperature at or above about 50 degrees Celsius.

4. The isolated nucleic acid sequence of claim 1 , wherein the nucleic acid sequence is present in a vector.

5. The isolated nucleic acid sequence of claim 1 , wherein the polypeptide has activity as a Type II restriction-modification system methylation subunit.

6. The isolated nucleic acid sequence of claim 1 , wherein the polypeptide comprises SEQ ID NO: 2976.

7. The isolated nucleic acid sequence of claim 1 , wherein the nucleic acid sequence comprises SEQ ID NO: 2977.

Continuity (2)
Division 12800045 · May 5, 2010
Related Publication 20130029400A1 · Jan 31, 2013