Lipoparticles comprising proteins, methods of making, and using the same
The present invention relates to lipoparticles. The invention also relates to producing lipoparticles. The invention further relates to lipoparticles comprising a viral structural protein. The invention further relates to a lipoparticle comprising a membrane protein, and the lipoparticle can be attached to a sensor surface. The invention further relates to methods of producing and using the lipoparticle to, inter alia, assess protein binding interactions.
1. A lipoparticle comprising
an external lipid bilayer;
an enveloped retroviral structural protein; and
a multiple membrane spanning protein,
wherein said enveloped retroviral structural protein is an uncleaved gag protein and wherein the multiple membrane spanning protein that is incorporated into the lipoparticle does not bind to the gag protein,
provided that the only viral proteins in the lipoparticle are structural proteins.
2. The lipoparticle of claim 1 wherein said multiple membrane spanning protein is a G protein coupled receptor.
3. The lipoparticle of claim 1 , wherein said Gag is from murine leukemia virus, rous sarcoma virus, HIV, SIV, avian leukemia virus, or equine anemia virus.
4. A composition comprising an isolated lipoparticle of claim 1 attached to a biosensor surface.
5. The lipoparticle of claim 1 , further comprising a G-protein.
6. The lipoparticle of claim 5 , wherein said G-protein is a modified G-protein.
7. The lipoparticle of claim 6 , wherein said modified G-protein comprises a fusion protein.
8. The lipoparticle of claim 7 , wherein said fusion protein comprises a fluorescent protein, a linker, a viral protein, membrane protein, a protease cleavage sequence, or combinations thereof.
9. The lipoparticle of claim 2 , wherein said G protein coupled receptor (GPCR) is a modified GPCR.
10. The lipoparticle of claim 9 , wherein said modified GPCR comprises a fusion protein.
11. The lipoparticle of claim 10 , wherein said fusion protein comprises a fluorescent protein, a linker, a viral protein, membrane protein, a protease cleavage sequence, or combinations thereof.
12. The lipoparticle of claim 5 further comprising a GTP analog.
13. The lipoparticle of claim 12 wherein said GTP analog is a fluorescent GTP analog.
14. An immunogen comprising a lipoparticle according to claim 1 .
15. A method of eliciting an immune response to a multiple membrane spanning protein, said method comprising the introduction of the lipoparticle of claim 1 to an animal.
16. The lipoparticle of claim 1 , wherein said multiple membrane spanning protein is an ion channel protein.
17. The lipoparticle of claim 1 , wherein said multiple membrane spanning protein is a transporter protein.
18. A lipoparticle comprising
an external lipid bilayer;
an enveloped retroviral structural protein; and
a multiple membrane spanning protein,
wherein said enveloped retroviral structural protein is an unmodified, uncleaved gag protein and, provided that the only viral proteins in the lipoparticle are structural proteins.
19. A lipoparticle comprising
an external lipid bilayer;
an enveloped retroviral structural protein; and
a multiple membrane spanning protein,
wherein said enveloped retroviral structural protein is an uncleaved gag protein and wherein said gag protein does not comprise a heterologous tag,
provided that the only viral proteins in the lipoparticle are structural proteins.