Fermentation process
Provided is a fermentation process wherein green ripe uncured vanilla beans are incubated with bacillus subtilis in isolated form to convert glucovanillin to vanillin while consistently forming a fully developed well-balanced vanilla aroma without off-notes.
1. A process of fermenting vanilla beans comprising the step of incubating uncured vanilla beans with Bacillus subtilis in isolated form, wherein the incubation with B. subtilis lasts for about 24 hours to about 120 hours, and wherein before incubation with B. subtilis , the uncured vanilla beans are dried until a moisture concentration of less than 10% is reached, and then treated with a water containing composition comprising Bacillus subtilis.
2. The process of claim 1 wherein the incubation with B. subtilis is performed at a temperature from about 40° C. to about 65° C.
3. The process of claim 1 , wherein before being dried, the uncured vanilla beans are washed.
4. The process of claim 1 , wherein after the incubation with B. subtilis , one or more enzymes comprising a beta-glucosidase enzyme are added.
5. The process of claim 1 wherein a solvent is added to the resulting product to form a vanilla extract.
6. The process of claim 1 wherein the incubation with B. subtilis is performed in a vessel selected from the group consisting of: a sweating tank wherein the vanilla beans are stationary, and a stirred tank.
7. The process of claim 6 wherein the incubation with B. subtilis is performed in a closed system of a sweating tank wherein the vanilla beans are stationary and wherein the exudate from the vanilla beans is being recycled.
8. The process of claim 1 wherein the incubation with B. subtilis is performed in an open system.
9. The process of claim 1 , wherein B. subtilis is selected from the group consisting of B. subtilis subtilis , a wildtype B. subtilis strain isolated from vanilla beans, and B. subtilis subtilis strain NRRL B-50118.
10. A process according to claim 1 , wherein the B. subtilis is provided in isolated form.
11. A process according to claim 1 , wherein the B. subtilis used in the fermentation step is provided from a stock culture or as a pre-culture.
12. The process according to claim 3 , wherein the beans are washed in a temperature of between 20-85° C.
13. The process according to claim 3 , wherein the beans are washed for between 1-10 minutes.