Methods for isolation, use and analysis of ferritin
This invention provides methods of isolating ferritin from plant and animal material. The isolated ferritin can be administered to humans or animals in need of iron, and can be used to treat or supplement iron deficiency. The isolated ferritin can be used in industrial applications, such as increasing the iron content in heat-processed food or beverages. The methods of the invention also include quantitation of iron derived from plant or animal ferritin.
1. A method to isolate ferritin from plant material comprising:
(a) separation of the plant material into a soluble and insoluble fraction;
(b) removal of non-ferritin components from the soluble fraction by enzymatic treatment of the soluble fraction with one or more carbohydrate-degrading enzymes, thereby isolating ferritin; and
(c) concentration of the isolated ferritin from the soluble fraction.
2. The method of claim 1 wherein the plant material comprises legumes.
3. The method of claim 2 wherein the legumes comprise soybeans.
4. The method of claim 1 wherein the plant material comprises the waste stream from the processing of legumes.
5. The method of claim 1 further comprising the step of fractionation of the soluble fraction.
6. The method of claim 1 further comprising the step of treatment of the soluble fraction with a solvent.
7. The method of claim 6 wherein the solvent is an organic solvent.
8. The method of claim 1 wherein the plant material comprises the entire plant.
9. The method of claim 1 wherein the plant material is selected from the group comprising a seed, a bean, a stem, a fruit, a leaf, a root, a flower and combinations of the preceding.
10. The method of claim 1 wherein the step of soluble and insoluble separation comprises mechanical separation.
11. The method of claim 1 wherein concentration of the isolated ferritin comprises drying the soluble fraction.
12. The method of claim 1 wherein concentration of the isolated ferritin comprises ultrafiltration.
13. The method of claim 1 wherein the separation of the plant material into a soluble and insoluble fraction comprises extraction of the plant material.