Peptide-coated cell localization to diseased or damaged tissues and methods related thereto
Embodiments of the present invention are directed to a coated cell comprising a therapeutic cell and a plurality of targeting complexes coating the therapeutic cell and each of said targeting complexes comprising a homing molecule, a lipid moiety, and a spacer having from about 1 to about 10 amino acids and covalently linking the homing molecule to the lipid moiety and wherein the lipid moiety is non-covalently attached to the therapeutic cell. In some embodiments, the therapeutic cell is a stem cell. Embodiments of the invention are directed to methods of coating a therapeutic cell. Embodiments of the invention are directed to methods of treating diseases of the vasculature.
1. A targeting complex comprising:
a heart homing peptide, CRPPR (SEQ ID NO: 1);
a palmitate moiety, linked to the amino terminus of a spacer comprising amino acids chosen from NKS and NSK wherein
the spacer covalently links the homing peptide to the lipid moiety and further comprising a cell.
2. The targeting complex of claim 1 , wherein the homing molecule selectively homes to vasculature.
3. A coated cell comprising:
a cell; and
a plurality of targeting complexes coating the cell;
each of said targeting complexes comprising:
a heart homing peptide, CRPPR (SEQ ID NO: 1);
a palmitate moiety linked to the amino terminus of a spacer comprising amino acids chosen from NKS and NSK and to the cell; wherein
the spacer covalently links the homing peptide to the palmitate moiety.
4. The coated cell of claim 3 , wherein the lipid moiety is non-covalently attached to the cell.
5. The coated cell of claim 3 , wherein the lipid moiety is integrated into a lipid bilayer of a cell membrane of the cell.
6. The coated cell of claim 3 , wherein the lipid moiety intercalates into a lipid bilayer of a cell membrane of the cell.
7. The coated cell of claim 3 , wherein the targeting complex is present on the surface of the cell at a concentration of from about 0.001 μM to about 1 mM.
8. A pharmaceutical composition comprising:
a cell;
a plurality of targeting complexes coating the cell;
each of said targeting complexes comprising:
a heart homing peptide, CRPPR (SEQ ID NO: 1);
a palmitate moiety linked to the amino terminus of a spacer comprising amino acids chosen from NKS and NSK; wherein the spacer covalently links the homing peptide to the palmitate moiety; and
a pharmaceutically acceptable carrier.
9. The pharmaceutical composition of claim 8 , wherein the lipid moiety is non-covalently attached to the cell.
10. The pharmaceutical composition of claim 8 , wherein the lipid moiety is integrated into a lipid bilayer of a cell membrane of the cell.
11. The pharmaceutical composition of claim 8 , wherein the lipid moiety intercalates into a lipid bilayer of a cell membrane of the cell.
12. The targeting complex of claim 1 , wherein the cell is a multi-potent progenitor cell.
13. The targeting complex of claim 1 , wherein the cell is a mesenchymal stem cell.
14. The targeting complex of claim 1 , wherein the cell is a hematopoietic stem cell.
15. The coated cell of claim 3 , wherein the cell is a multipotent progenitor cell.
16. The coated cell of claim 3 , wherein the cell is a mesenchymal stem cell.
17. The coated cell of claim 3 , wherein the cell is a hematopoietic stem cell.
18. The pharmaceutical composition of claim 8 , wherein the cell is a multipotent progenitor cell.
19. The pharmaceutical composition of claim 8 , wherein the cell is a mesenchymal stem cell.
20. The pharmaceutical composition of claim 8 , wherein the cell is a hematopoietic stem cell.