cDNA-derived nucleic acids encoding red-shifted channelrhodopsins
Methods and compositions are used to identify and characterize new channelrhodopsins derived from algae and several of which are red-shifted. The rhodopsin domain of these red-shifted channelrhodopsins can be cloned and expressed in mammalian systems and used in optogenetic applications and as therapeutic agents. Also provided are methods and compositions for use in red-shifting the absorbance maxima of channelrhodopsins in order to improve their utility for use in vivo.
1. A cDNA-derived nucleic acid comprising a nucleic acid sequence that encodes an amino acid sequence consisting of SEQ ID NO: 1.
2. The cDNA-derived nucleic acid of claim 1 , wherein the cDNA-derived nucleic acid encodes the amino acid sequence of SEQ ID NO: 1.
3. An expression vector comprising the cDNA-derived nucleic acid of claim 1 .