Modified human immunodeficiency virus clade C envelope polypeptides obtained from a zambian isolate
The invention provides methods and compositions for raising an immune response in a subject by administering an HIV antigen. The HIV antigens include HIV clade C polynucleotides and polypeptides. The invention also provides for recombinant HIV viral particles and compositions.
1. An isolated HIV envelope polypeptide, wherein said polypeptide comprises an amino acid sequence selected from the group consisting of SEQ ID NOS.:2, 4, 6, 8 and 10.
2. A composition comprising said HIV envelope polypeptide of claim 1 and a pharmacologically acceptable carrier.
3. An isolated polynucleotide encoding the HIV envelope polypeptide of claim 1 .
4. A construct comprising said HIV envelope polynucleotide of claim 3 .
5. A composition comprising said HIV envelope polynucleotide of claim 4 and a pharmacologically acceptable carrier.
6. A method for inducing an anti-human immunodeficiency virus (HIV) immune response in a subject in need thereof, wherein said method comprises administering an immunogenic composition comprising the polypeptide of claim 1 to said subject.
7. The method of claim 6 , further comprising administering a second HIV antigen.
8. The method of claims 7 , wherein said second HIV antigen is Gp120 or gp160.
9. The method of claim 6 , wherein said immune response treats an HIV infection.
10. An HIV recombinant viral particle comprising the polypeptide of claim 1 .
11. The HIV recombinant viral particle of claim 10 , wherein said viral particle further comprises the Nef amino acid sequence of SEQ ID NO:11.
12. The recombinant viral particle of claim 10 , wherein said viral particle comprises two or more NF-κB regions.