Temperature control device with a flexible temperature control surface
A device for controlling temperature in a reaction chamber is disclosed. The device comprises: a bladder assembly comprising a housing dimensioned to hold a reaction chamber disposed within an interior volume of the housing; and a first temperature-control bladder disposed within the housing, the first temperature-control bladder is configured to receive a temperature-control fluid and comprises a flexible, heat conductive surface that comes in contact with at least a portion of an exterior surface of the reaction chamber after receiving the temperature-control fluid. Also disclosed are a bladder thermal cycler, a temperature-control bladder assembly and methods for producing a thermal cycle in a reaction chamber.
1. A method for performing a polymerase chain reaction (PCR) in a reaction chamber, comprising:
(a) placing said reaction chamber in the vicinity of a temperature-control bladder having a flexible thermal conductive surface, said thermal conductive surface forms a contact with an exterior surface of said reaction chamber when said temperature-control bladder is filled with a temperature control fluid, wherein said flexible thermal conductive surface facilitates heat transfer from said temperature control fluid to said reaction chamber;
(b) filling said temperature-control bladder with said temperature-control fluid at a first temperature;
(c) holding said temperature-control fluid at the first temperature in said temperature-control bladder for a first period of time;
(d) filling said temperature-control bladder with said temperature-control fluid at a second temperature; and
(e) holding said temperature-control fluid at the second temperature in said temperature-control bladder for a second period of time.
2. The method of claim 1 , further comprising:
filling said temperature-control bladder with said temperature-control fluid at a third temperature; and
holding said temperature-control fluid at the third temperature in said temperature-control bladder for a third period of time.
3. The method of claim 1 , wherein said temperature-control fluid at the first temperature is produced at a first heat exchanger and is re-circulated back to said first heat exchanger after step (c), and wherein said temperature-control fluid at the second temperature is produced at a second heat exchanger and is re-circulated back to said second heat exchanger after step (e).
4. A method for producing thermal cycling in a reaction chamber having thermal contact with a temperature-control bladder, comprising:
(a) filling said temperature-control bladder with a temperature-control fluid at a first temperature and holding said temperature-control fluid in said temperature-control bladder for a first period of time;
(b) filling said temperature-control bladder with said temperature-control fluid at a second temperature and holding said temperature-control fluid in said temperature-control bladder for a second period of time; and
(c) repeating steps (a) and (b),
wherein said reaction chamber is a PCR tube or a microarray slide and wherein said temperature-control bladder comprises a flexible thermal conductive surface that facilitates heat transfer from said temperature control fluid to said reaction chamber.
5. The method of claim 1 , wherein said reaction chamber is a PCR tube or a microarray slide.
6. The method of claim 1 , wherein said first temperature is in the range of 80-105° C. and wherein said second temperature is in the range of 50-70° C.
7. The method of claim 6 , wherein said first temperature is about 95° C. and wherein said second temperature is about 65° C.
8. The method of claim 1 , wherein steps (b)-(e) are repeated for 30-50 times.
9. The method of claim 1 , wherein said temperature-control bladder is capable of heating a reaction mixture in said reaction chamber at a rate of about 15° C./sec.
10. The method of claim 4 , wherein said temperature-control fluid at the first temperature is produced at a first heat exchanger and is re-circulated back to said first heat exchanger after step (a), and wherein said temperature-control fluid at the second temperature is produced at a second heat exchanger and is re-circulated back to said second heat exchanger after step (b).
11. The method of claim 4 , wherein said first temperature is in the range of 80-105° C. and wherein said second temperature is in the range of 50-70° C.
12. The method of claim 11 , wherein said first temperature is about 95° C. and wherein said second temperature is about 65° C.
13. The method of claim 4 , wherein steps (a) and (b) are repeated for 30-50 times.
14. The method of claim 4 , wherein said temperature-control bladder is capable of heating a reaction mixture in said reaction chamber at a rate of about 15° C./sec.