Panel for the detection and differentiation of renal cortical neoplasms
The present invention provides a novel, highly sensitive and specific probe panel which detects the type of renal cortical neoplasm present in a biopsy sample. As such, the invention permits diagnosis of the predominant subtypes of renal cortical neoplasms without the use of invasive methods. The present invention further provides a molecular cytogenetic method for detecting and analyzing the type of renal cortical neoplasm present in a renal biopsy sample.
1. A panel for detecting the type of renal cortical neoplasm present in a sample, wherein said panel comprises a plurality of labeled probes each of which is individually capable of hybridizing selectively to a specific marker associated with a chromosomal abnormality diagnostic or indicative of the type of renal cortical neoplasm, and wherein said plurality of labeled probes comprises:
a probe that is capable of hybridizing selectively to, 5′TFE3;
a probe that is capable of selectively hybridizing to 3′TFE3;
a probe that is capable of selectively hybridizing to the marker identified as D3S4208;
a probe that is capable of selectively hybridizing to the marker identified as VHL;
a probe that is capable of selectively hybridizing to the marker identified as D3S3634;
a probe that is capable of selectively hybridizing to 7 α-satellite;
a probe that is capable of selectively hybridizing to the marker identified as D17S651;
a probe that is capable of selectively hybridizing to the marker identified as D3S1212;
a probe that is capable of selectively hybridizing to the marker identified as D6S1941;
a probe that is capable of selectively hybridizing to the marker identified as D6S2419;
a probe that is capable of selectively hybridizing to the marker identified as D10S1765;
a probe that is capable of selectively hybridizing to the marker identified as D1S2471;
a probe that is capable of selectively hybridizing to the marker identified as D14S1007;
a probe that is capable of selectively hybridizing to Y satellite III;
a probe that is capable of selectively hybridizing to 5′CCND1;
a probe that is capable of selectively hybridizing to 3′CCND1;
a probe that is capable of selectively hybridizing to the marker identified as D5S2095;
a probe that is capable of selectively hybridizing to the marker identified as D5S1469; and
a control probe that is capable of being used to assess ploidy in the sample;
wherein each of said labeled probes comprises a moiety selected from the group consisting of a radioactive moiety, a fluorogenic moiety, a chromogenic moiety, and an enzymatic moiety.
2. The panel of claim 1 , wherein said sample is a renal biopsy sample.
3. The panel of claim 1 , wherein said control probe is the marker identified as D22S553.
4. The panel of claim 1 , wherein each of said labeled probes comprises a label, said label comprising a substance that can be attached to a probe so that when the probe binds to the marker a signal is emitted or the labeled probe can be detected by a human observer or an analytical instrument.