Apparatus for solubilizing tissue
Tissue solubilizing apparatuses are provided. The apparatuses may be useful to solubilize tissue, including skin, mucosal membrane, and other tissue. The apparatuses may be further useful to preserve and recover analytes contained within the solubilized skin, mucosal membrane, and other tissue.
1. An apparatus for solubilizing tissue, comprising:
a housing, comprising a vacuum chamber and a passage,
wherein the vacuum chamber comprises an outer wall, an inner wall, an upper wall, and an interior,
wherein the outer wall, the inner wall, and the upper wall define the interior, and wherein the inner wall is radially inward of the outer wall,
wherein the vacuum chamber is configured to selectively generate an air pressure within the interior that is less than ambient air pressure,
wherein the interior is configured to be operatively connected to the tissue,
wherein the passage is configured to be operatively connected to the tissue;
an energy source configured to be operatively connected to the tissue to apply energy to the tissue,
wherein the energy source is configured to access the tissue through the passage; and
a reservoir housing configured to contain a liquefaction promoting medium, the reservoir housing being operatively connected to apply the liquefaction promoting medium to the tissue,
the reservoir housing and the energy source being operatively connected to solubilize the tissue with the liquefaction promoting medium and the energy from the energy source.
2. The apparatus of claim 1 , further comprising a port operatively connected to the vacuum chamber, wherein the port is configured to selectively allow at least one of introduction of a liquefaction promoting medium into the vacuum chamber and removal from the vacuum chamber of a liquefaction promoting medium containing solubilized tissue.
3. The apparatus of claim 1 , further comprising a port operatively connected to the passage, wherein the port is configured to selectively allow at least one of introduction of liquefaction promoting medium into the passage and removal from the vacuum chamber of a liquefaction promoting medium containing solubilized tissue.
4. The apparatus of claim 1 , further comprising a sample container, at least a portion of which is positioned within at least a portion of the passage.
5. The apparatus of claim 4 , further comprising a port operatively connected to the sample container, wherein the port is configured to at least one of: selectively allow introduction of liquefaction promoting medium into the vacuum chamber and removal from the vacuum chamber of a liquefaction promoting medium containing solubilized tissue.
6. The apparatus of claim 1 , wherein the energy source is configured to apply the energy as a mechanical energy to the tissue.
7. The apparatus of claim 1 , wherein the energy source comprises an abrasive component connected to a shaft, and wherein the abrasive component is at least one of: a sheet of abrasive material, an abrasive disc, an abrasive ring, and a brush having bristles.
8. The apparatus of claim 1 , wherein at least a portion of the outer wall is flexible and biased toward a radially outward direction, and wherein at least a portion of the outer wall is configured to be selectively displaced radially inwardly and released, resulting in the generation of an air pressure within the interior that is less than ambient air pressure.