Assay
RAN and RAN Binding Protein 1 have been determined to be markers of invasive and metastatic potential of a tumour cell. There is described methods and kits for the detection of the level of RAN and RAN Binding Protein 1 and the use thereof.
1. A method of determining whether a tumour cell is likely to have an increased incidence of metastasis comprising the steps:
determining the level of a marker in a tumour cell from a sample, wherein the marker is at least one member of the group comprising the RAN polypeptide of SEQ ID NO 1, the RAN Binding Protein 1 of SEQ ID NO 3, an active fragment of said RAN polypeptide, an active fragment of said RAN Binding Protein 1, a nucleic acid sequence encoding said RAN polypeptide, a nucleic acid sequence encoding said RAN Binding Protein 1, a nucleic acid sequence encoding an active fragment of said RAN polypeptide, and a nucleic acid sequence encoding an active fragment of said RAN Binding Protein 1 and
comparing the level of the marker detected with the level determined for the marker in a non-invasive and/or non-metastatic tumour cell,
wherein an increase in the level of the marker in the tumour cell from the sample is indicative that the tumour cell is likely to have an increased incidence of metastasis.
2. A method for monitoring a tumour to determine the invasive and/or metastatic potential of said tumour, the method comprising the steps of:
a) detecting in a tumour cell at a first time point, the level of a marker, wherein the marker is at least one member of the group comprising the RAN polypeptide of SEQ ID NO 1, the RAN Binding Protein 1 of SEQ ID NO 3, an active fragment of said RAN polypeptide, an active fragment of said RAN Binding Protein 1, a nucleic acid sequence encoding said RAN polypeptide, a nucleic acid sequence encoding said RAN Binding Protein 1, a nucleic acid sequence encoding an active fragment of said RAN polypeptide, and a nucleic acid sequence encoding an active fragment of said RAN Binding Protein 1,
b) repeating step a) at a subsequent point in time; and
c) comparing the level of a marker in steps a) and b) wherein an increase in the level of marker from a first time point to a subsequent time point is indicative that the tumour cell is gaining invasive and/or metastatic potential.
3. The method as claimed in claim 1 wherein the level of the marker is determined by detecting the binding of an antibody, antibody derivative or antibody fragment with binding specificity to at least one of the RAN polypeptide of SEQ ID NO 1, the RAN Binding Protein 1 of SEQ ID NO 3, an active fragment of said RAN polypeptide, and an active fragment of said RAN Binding Protein 1.
4. The method as claimed in claim 2 wherein the level of the marker is determined by detecting the binding of an antibody, antibody derivative or antibody fragment with binding specificity to at least one of the RAN polypeptide of SEQ ID NO 1, the RAN Binding Protein 1 of SEQ ID NO 3, an active fragment of said RAN polypeptide, and an active fragment of said RAN Binding Protein 1.