Multispectral illumination device
An illumination device includes at least four semiconductor radiation sources ( 18 ) for emitting optical radiation in respectively different emission wavelength ranges. At least one color splitter ( 22.1, 22.2, 22.3 ), which is reflective for optical radiation of the respective semiconductor radiation source ( 18 ), is assigned to each of at least three of the semiconductor radiation sources ( 18 ). The semiconductor radiation sources ( 18 ) and the color splitters ( 22.1, 22.2, 22.3 ) are arranged such that the optical radiation, which is emitted in each case from each of the semiconductor radiation sources ( 18 ), is coupled into a common illumination beam path section ( 24 ). In each case, one collimating unit ( 20.1, 20.2, 20.3, 20.4 ), which collimates the optical radiation emitted by the respective semiconductor radiation source ( 18 ), is arranged in the beam path sections from the semiconductor radiation sources ( 18 ) to the color splitters ( 22.1, 22.2, 22.3 ).
1. An optical arrangement configured to examine a specimen comprising:
an optical assembly defining an optical axis;
an illuminating device for supplying optical radiation along an illuminating beam path;
means for coupling said optical radiation into said optical assembly for transmission along said optical axis; and,
said illuminating device including:
at least two light emitting diodes for emitting optical radiation with respectively different emission spectra and along respective beam path segments;
said means for coupling including at least one color splitter assigned to each of said light emitting diodes, respectively;
each of said color splitters being reflective for the optical radiation of the light emitting diode corresponding thereto;
said light emitting diodes and said color splitters being so arranged that the optical radiation emitted by each of said light emitting diodes is coupled into a common illuminating beam path segment;
a plurality of collimating units mounted in said beam path segments, respectively, to collimate the optical radiation emitted by corresponding ones of said light emitting diodes and passing to respective ones of said color splitters;
said optical arrangement being configured to conduct fluorescence examinations of said specimen;
said optical assembly defining a detection beam path along which fluorescence radiation emanating from said specimen travels;
said detection beam path for said fluorescence radiation including at least one multiband emission filter or multiband emission filter set;
wherein at least one of said emission spectra of said light emitting diodes lies with its characteristic wavelength between two emission wavelength ranges of said multiband emission filter or said multiband emission filter set; and,
said optical assembly further comprising a white-light emitting diode being coupled into said common illuminating beam path by a partially transmitting deflecting mirror;
wherein the optical radiation emitted by each of said light emitting diodes is reflected by said partially transmitting deflecting mirror and the optical radiation emitted by said white-light emitting diode is transmitted through said partially transmitting deflecting mirror.
2. The optical arrangement of claim 1 , wherein the collimating units are aspheres.
3. The optical arrangement of claim 2 , wherein positions of the collimating units are adjustable relative to the respective light emitting diodes.
4. The optical arrangement of claim 1 , wherein said optical arrangement is configured as a wide field microscope or a fluorescence reader.
5. The optical arrangement of claim 1 , wherein said multiband emission filter is contained in a filter cube and,
wherein said optical arrangement further includes a multiband excitation filter or multiband excitation filter set and a multiband color splitter for deflecting said optical radiation of said illuminating device or said fluorescence radiation emanating from said specimen;
said multiband excitation filter or said multiband excitation filter set being mounted in said illuminating beam path of said illuminating device.
6. The optical arrangement of claim 5 , wherein said multiband excitation filter is a first multiband excitation filter and said optical arrangement further includes a second multiband excitation filter; and,
said multiband excitation filters have filter edges shifted relative to each other by a distance of between 10 nm and 90 nm.
7. The optical arrangement of claim 1 , further comprising a receptacle for at leapt two filter cubes by means of which one of the filter cubes is movable into said detection beam path.