Composition for enhancing immunity containing plant stem cell line derived from cambium of
View Patent ↗The present invention relates to a composition for enhancing immunity, comprising one or more of the following: a homogenous cell line, and a lysate, an extract and a culture medium thereof as an active ingredient. The homogenous cell line, the lysate, the extract and the culture thereof according to the present invention, which are derived from a natural product, minimize adverse side effects of prior immune enhancing agents and safe for the human body. Further, they effectively increase the activity of NK cells responsible for innate immunity, as well as increase the proliferation rate of lymph node cells when the cells were re-exposed to an antigen in a specific immune reaction to enhance acquired immunity, and thus are useful as an immune enhancing agent. In particular, the homogenous cell line, the lysate, the extract, and the culture thereof according to the present invention also effectively increase the number of hone marrow cells, thus are not only used as an adjuvant in an immune reaction, but also used in the prevention and treatment of anemia through hematopoiesis.
1. A method for enhancing immunity in a mammal, comprising administering to the mammal one or more of the following: a cell line, a lysate of the cell line, an extract of the cell line, and a culture of the cell line, wherein the cell line is: derived from the cambium of Panax ginseng, is in an innately undifferentiated state, and is not callus.
2. The method of claim 1 , wherein the cell line is present as single cell level during a suspension culture.
3. The method of claim 1 , wherein the cell line is obtained by using an isolation method comprising the steps of: (a) obtaining a Panax ginseng cambium-containing storage root tissue; (b) culturing the cambium-containing storage root tissue in a medium supplemented with indole-3-acetic acid or indole-3-butyric acid to induce a cell line derived from the cambium, wherein an osmotic stress is applied to the cambium-containing storage root tissue before, during, or after the culture; and (c) collecting the induced, cambium-derived cell line.
4. The method of claim 3 , which further comprises culturing the cell line collected in step (c) in a medium supplemented with an elicitor and collecting the cell line.
5. The method of claim 3 , wherein step (c) is performed by proliferating the induced cambium-derived cell line in a medium which contains one or more of 2,4-dichlorophenoxyacetic acid, picloram and IBA, and then collecting the cambium-derived cell line.
6. The method of claim 4 , wherein the elicitor is raw sugar or methyl jasmonate.
7. The method of claim 1 , wherein the extract is obtained by extracting the cell line with a solvent selected from the group consisting of distilled water, alcohol, acetone, dimethyl sulfoxide and a mixture thereof.
8. The method of claim 1 , wherein any one among the cell line and the lysate, the extract and the culture thereof has an immune enhancing effect by increasing the activity of NK cells.
9. The method of claim 1 , wherein any one among the cell line and the lysate, the extract and the culture thereof has an immune enhancing effect by increasing the number of lymph node cells specific for an antigen.