Cultured hematopoietic stem cells and method for expansion and analysis thereof
View Patent ↗Hematopoietic stem cells and methods for ex vivo expansion of hematopoietic stem cells are provided. The methods comprise culturing the cells in a media containing an effective amount insulin-like growth factor(IGF), fibroblast growth factor (FGF), thrombopoietin (TPO), and stem cell factor (SCF), under conditions sufficient for expansion of said cells. Methods for identifying expanded hematopoeitc stem cells and kits for ex vivo expansion of hematopoietic stem cells are also provided.
1. A method for expanding hematopoietic stem cells ex vivo, comprising culturing a population of cells that includes hematopoietic stem cells in a medium comprising insulin-like growth factor 2 (IGF-2) and stem cell factor (SCF), under conditions sufficient for expansion of the hematopoietic stem cells, thereby expanding hematopoietic stem cells ex vivo and enriching for hematopoietic stem cells relative to the initial population of cells.
2. The method of claim 1 , wherein the medium further comprises at least one of fibroblast growth factor (FGF) and thrombopoietin (TPO).
3. The method of claim 2 , wherein both FGF and TPO are present in the medium.
4. The method of claim 2 , wherein the FGF is FGF-1.
5. The method of claim 2 , wherein the medium comprises IGF-2, FGF-1, SCF, and TPO.
6. The method of claim 1 , wherein the IGF-2 is present at a concentration of about 20 ng/ml.
7. The method of claim 2 , wherein FGF is present at a concentration of about 10 ng/ml.
8. The method of claim 2 , wherein TPO is present at a concentration of about 20 ng/ml.
9. The method of claim 1 , wherein SCF is present at a concentration of about 10 ng/ml.
10. The method of claim 1 , wherein the population of cells are cultured for about two weeks.
11. The method of claim 1 , wherein said population of cells comprises total bone marrow.
12. The method of claim 1 , wherein said population of cells comprises a sub-population of total bone marrow cells.
13. The method of claim 1 , wherein the expanded hematopoietic stem cells are at least about 8-fold enriched for long term hematopoietic stem cells.
14. The method of claim 1 , wherein said culture medium comprises serum-free medium.
15. A kit for expanding and enriching for hematopoietic stem cells ex vivo comprising a) insulin-like growth factor 2 (IGF-2), stem cell factor (SCF), thrombopoietin (TPO), and fibroblast growth factor 1 (FGF-1); and b) instructions for expanding hematopoietic stem cells ex vivo and for enriching for hematopoietic stem cells relative to an initial population of cells that includes hematopoietic stem cells.
16. The kit of claim 15 , where the IGF-2, SCF, TPO and FGF-1 are provided in separate containers.
17. A method for expanding hematopoietic stem cells ex vivo, comprising culturing a population of cells that includes hematopoietic stem cells in a serum-free medium comprising insulin-like growth factor-2 (IGF-2), fibroblast growth factor 1 (FGF-1), thrombopoietin (TPO), and stem cell factor (SCF), under conditions sufficient for expansion of the hematopoietic stem cells, thereby expanding hematopoietic stem cells ex vivo.