Actuated septa and systems and methods using the same
A device, system, and method for passively sealing a vessel containing a fluid and for sampling the fluid without carry-over or cross-contamination between the fluid sampling device, the sealing septum, and the vessel contents. The device includes an actuated septum having a plurality of septum fingers, to passively seal the vessel, and an actuation device, to open the passive seal without carry-over or cross-contamination. Each of the plurality of septum fingers includes a corresponding rib portion. The actuation device can be an actuation ring having an annulus. The plurality of septum fingers and corresponding rib portions are disposed internal or substantially internal to the vessel, while the actuation device is disposed external to the vessel.
1. A system for sealing a vessel containing a fluid and for providing contamination-free sampling of the fluid from the vessel, the vessel having an opening into the vessel interior, the system comprising:
a fluid transfer device for adding to or removing the fluid from the vessel;
an actuatable septum for sealing the vessel, the septum comprising:
an outer ring portion to provide a tight, interference seal between the actuatable septum and an outer surface of the opening of the vessel, and
at least three septum fingers that are cantilevered from the outer ring portion, the septum fingers in a natural state producing a passive seal, each of the septum fingers having a corresponding upwardly projecting rib portion, the septum fingers and corresponding upwardly projecting rib portions being disposed internal or substantially internal to the vessel;
a substantially ring-shaped actuation device disposed external to the vessel and adapted for selective engagement with the upwardly projecting rib portions of the septum fingers, wherein engagement between the actuation device and the upwardly projecting rib portions of the septum fingers results in a deflection of the septum fingers; and
a flexible, skirt-like neck portion encircling and connecting the actuation device with the outer ring portion.
2. The system as recited in claim 1 , wherein the fluid transfer device is selected from the group consisting of a hollow annulus or a sampling probe.
3. The system as recited in claim 1 , wherein the fluid transfer device and actuation device are structured and arranged coaxially with the fluid transfer device disposed inside of an annulus of the actuation device.
4. The system as recited in claim 1 , further comprising:
means for depressing the actuation device against the upwardly projecting ribs of the septum fingers, to break the passive seal and to provide an opening into the vessel through the actuatable septum.
5. The system as recited in claim 4 , further comprising:
control means for controlling the means for depressing the actuation device and for introducing, advancing, and withdrawing the fluid transfer device into or from the vessel.
6. A method of sampling a fluid from a passively sealed vessel with reduced carry-over or cross contamination, the vessel having an opening into the vessel interior, the method comprising:
sealing the vessel with an actuatable septum having a central axis, the actuatable septum comprising:
an outer ring portion to provide a tight, interference seal between the actuatable septum and an outer surface of the opening of the vessel, and
at least three septum fingers that are cantilevered from the outer ring portion, the septum fingers in a natural state producing a passive seal, each of the septum fingers having a corresponding upwardly projecting rib portion, the septum fingers and corresponding upwardly projecting rib portions being disposed internal or substantially internal to the vessel;
depressing a substantially ring-shaped actuation device connected to the outer ring portion by a flexible, skirt-like neck portion, the actuation device being disposed external to the vessel and adapted for selective engagement with the upwardly projecting rib portions of the septum fingers, against said upwardly projecting rib portions of the septum fingers, causing said septum fingers to deflect, to provide an opening in the actuatable septum;
introducing a fluid sampling device through an annulus in the actuation device and the opening in the actuatable septum;
sampling the fluid through the fluid sampling device;
removing the fluid sampling device from the opening in the actuatable septum; and
removing the actuation device from against the upwardly projecting rib portions, allowing the septum fingers to close and passively re-seal the vessel.
7. The method as recited in claim 6 , wherein depressing the actuation device includes translating the actuation device in the central axis of the actuatable septum mechanically, magnetically or electromechanically.