Glycoprotein cancer biomarker
View Patent ↗The invention relates to glycoproteins having a cancer-specific glycoform. Cancer-specific glycoforms are useful in diagnostics and therapeutics.
1. A method for evaluating the presence, absence, nature or extent of breast cancer or a precancerous condition of the breast, the method comprising:
providing a biological sample obtained from a subject, the biological sample comprising glycoproteins;
contacting the biological sample with a glycan-binding molecule specific for a glycan, under conditions that permit binding of the glycan-binding molecule to a glycoprotein comprising said glycan, wherein the glycan comprises a GlcNAc β(1,6) Man branched N-linked glycan;
contacting the glycoprotein with an anti-periostin antibody; and
detecting binding of the glycan-binding molecule and the anti-periostin antibody to the glycoprotein to determine the presence, absence or amount of a cancer-specific glycoform of periostin in the biological sample, wherein said cancer-specific glycoform comprises the glycan and is indicative of breast cancer or a precancerous condition of the breast; and wherein the presence, absence or amount of the cancer-specific glycoform is indicative of the presence, absence, nature or extent of breast cancer or a precancerous condition of the breast.
2. The method of claim 1 wherein the glycan-binding molecule comprises a detectable label.
3. The method of claim 1 wherein the glycan-binding molecule is selected from the group consisting of a lectin, a glycospecific antibody, a glycospecific aptamer, a glycospecific peptide, and a glycospecific small molecule.
4. The method of claim 3 wherein the lectin comprises leukoagglutinating phytohemagglutinin (L-PHA).
5. The method of claim 1 wherein the glycan comprises a branched N-linked glycan extended with N-acetyllactosamine.
6. The method of claim 1 wherein the biological sample comprises a biological fluid.
7. The method of claim 1 wherein the biological sample comprises serum or plasma.
8. The method of claim 1 wherein the biological sample comprises tissue.
9. The method of claim 1 wherein the subject is a human.
10. A method for evaluating the presence, absence, nature or extent of breast cancer or a precancerous condition of the breast, the method comprising:
contacting a biological sample obtained from a subject, with a glycan-binding molecule specific for a glycan under conditions that permit binding of the glycan-binding molecule to a glycoprotein comprising said glycan, wherein the glycan comprises a GlcNAc β(1,6) Man branched N-linked glycan;
contacting the glycoprotein with an anti-periostin antibody; and
detecting binding of the glycan-binding molecule and the anti-periostin antibody to the glycoprotein to determine the presence of a cancer-specific glycoform of periostin in the biological sample, wherein the cancer-specific glycoform comprises the glycan and is indicative of the presence of cancer or a precancerous condition.
11. A diagnostic composition comprising a biomarker comprising a isolated cancer-specific glycoform of periostin comprising a GlcNAc β(1,6) Man branched N-linked glycan component.