Treatment of pluripotent cells
The present invention is directed to methods to treat pluripotent cells, whereby the pluripotent cells can be efficiently expanded in culture and differentiated by treating the pluripotent cells with an inhibitor of GSK-3B enzyme activity.
1. A method to expand and differentiate human pluripotent cells, comprising the steps of:
a. Culturing human pluripotent cells, and
b. Treating the human pluripotent cells with an inhibitor of glycogen synthase kinase 3β(GSK-3B)enzyme activity consisting of 3-[1-(2- Hydroxyethyl)-1H-indol-3-yl]-4-(1-pyridin-3-yl-1H-indol-3-yl)-1H-pyrrole-2,5-dione.
2. The method of claim 1 , wherein the human pluripotent cells are embryonic stem cells.
3. The method of claim 1 , wherein the human pluripotent cells are cells expressing pluripotency markers of embryonic stem cells.
4. The method of claim 3 , wherein the cells expressing pluripotency markers express at least one of ATP-binding cassette, sub-family G, member 2 (ABCG2), crypto, forkhead box D3 (FoxD3), Connexin43, Connexin45, POU domain transcription factor Oct4, sex determining region Y-box 2 (SOX-2), Nanog, human telomerase reverse transcriptase (hTERT), undifferentiated embryonic cell transcription factor 1 (UTF-1), zinc finger protein 42 (ZFP42), stage-specific embryonic antigen 3 (SSEA-3), stage-specific embryonic antigen 4(SSEA-4), tumor-related antigen-1-60 (Tral-60), and tumor-related antigen-1-81 (Tral-81).
5. The method of claim 1 , wherein the pluripotent cells are differentiated into cells expressing markers characteristic of the definitive endoderm lineage.
6. The method of claim 1 , wherein the pluripotent cells are treated with the inhibitor of GSK-3B enzyme activity for about one to about 72 hours.
7. The method of claim 1 , wherein the pluripotent cells are treated with the inhibitor of GSK-3B enzyme activity for about 12 to about 48 hours.
8. The method of claim 1 , wherein the pluripotent cells are treated with the inhibitor of GSK-3B enzyme activity for about 48 hours.
9. The method of claim 1 , wherein the inhibitor of GSK-3B enzyme activity is used at a concentration of about 100 nM to about 100 μM.
10. The method of claim 1 , wherein the inhibitor of GSK-3B enzyme activity is used at a concentration of about 1 μM to about 10 μM.
11. The method of claim 1 , wherein the inhibitor of GSK-3B enzyme activity is used at a concentration of about 10 μM.