Assays
A method for assaying a sample for each of multiple analytes is described. The method includes contacting an array of spaced-apart test zones with a liquid sample (e.g., whole blood). The test zones disposed within a channel of a microfluidic device. The channel is defined by at least one flexible wall and a second wall which may or may not be flexible. Each test zone comprising a probe compound specific for a respective target analyte. The microfluidic device is compressed to reduce the thickness of the channel, which is the distance between the inner surfaces of the walls within the channel. The presence of each analyte is determined by optically detecting an interaction at each of multiple test zones for which the distance between the inner surfaces at the corresponding location is reduced. The interaction at each test zone is indicative of the presence in the sample of a target analyte.
1. A device for detecting an analyte, comprising:
a cartridge having:
a microfluidic channel including an inlet and a detection region in fluid communication with the inlet;
a microfluidic flow path having an at least partially deformable wall and in fluid communication with the detection region of the channel; and
a cap having: a sealing member configured to seal with the inlet and form a fluid circuit including the inlet, the microfluidic channel and the microfluidic flow path,
wherein the cap and cartridge are configured to close irreversibly after forming the fluid circuit.
2. The device of claim 1 , wherein the cap is flexibly attached to the cartridge.
3. The device of claim 1 , wherein the cap and cartridge are configured to engage in a first relative position such that the cap can be removed and to engage in a second relative position such that the cap is irreversibly closed after forming the fluid circuit.
4. The device of claim 1 , wherein the detection region is bounded by at least one surface of the cartridge and at least one surface of a lid.
5. The device of claim 4 , wherein the lid includes a transparent film over the detection region.
6. The device of claim 5 , wherein the lid is adhesively affixed to the cartridge.
7. A device for detecting an analyte, comprising:
a cartridge having:
a microfluidic channel including a capillary inlet having an anticoagulant on an inner surface, a chamber including a reagent, and a detection region in fluid communication with the inlet;
a microfluidic flow path having an at least partially deformable wall and in fluid communication with the detection region of the channel; and
a cap having:
a sealing member configured to seal with the inlet and form a fluid circuit including the inlet, the microfluidic channel and the microfluidic flow path,
wherein the cap and cartridge are configured to close irreversibly after forming the fluid circuit.
8. A device for detecting an analyte, comprising:
a cartridge having:
a microfluidic channel including an inlet and a detection region in fluid communication with the inlet;
a microfluidic flow path having an at least partially deformable wall and in fluid communication with the detection region of the channel; and
a cap having: a sealing member configured to seal with the inlet and form a fluid circuit including the inlet, the microfluidic channel and the microfluidic flow path,
wherein the cap is flexibly attached to the cartridge.
9. The device of claim 8 , wherein the cap and cartridge are configured to close irreversibly after forming the fluid circuit.
10. The device of claim 8 , wherein the cap and cartridge are configured to engage in a first relative position such that the cap can be removed and to engage in a second relative position such that the cap is irreversibly closed after forming the fluid circuit.
11. The device of claim 8 , wherein the detection region is bounded by at least one surface of the cartridge and at least one surface of a lid.
12. The device of claim 11 , wherein the lid includes a transparent film over the detection region.
13. The device of claim 12 , wherein the lid is adhesively affixed to the cartridge.
14. A device for detecting an analyte, comprising:
a cartridge having:
a microfluidic channel including an inlet and a detection region in fluid communication with the inlet;
a microfluidic flow path having an at least partially deformable wall and in fluid communication with the detection region of the channel; and
a cap having: a sealing member configured to seal with the inlet and form a fluid circuit including the inlet, the microfluidic channel and the microfluidic flow path,
wherein the detection region is bounded by at least one surface of the cartridge and at least one surface of a lid, the lid includes a transparent film over the detection region, and the lid is adhesively affixed to the cartridge.
15. The device of claim 14 , wherein the cap and cartridge are configured to close irreversibly after forming the fluid circuit.
16. The device of claim 14 , wherein the cap is flexibly attached to the cartridge.
17. The device of claim 14 , wherein the cap and cartridge are configured to engage in a first relative position such that the cap can be removed and to engage in a second relative position such that the cap is irreversibly closed after forming the fluid circuit.
18. A device for detecting an analyte, comprising:
a cartridge having:
a microfluidic channel including a capillary inlet having an anticoagulant on an inner surface, a chamber including a reagent, and a detection region in fluid communication with the inlet;
a microfluidic flow path having an at least partially deformable wall and in fluid communication with the detection region of the channel; and
a cap having:
a sealing member configured to seal with the inlet and form a fluid circuit including the inlet, the microfluidic channel and the microfluidic flow path,
wherein the cap is flexibly attached to the cartridge.