Anti-dengue virus antibodies
Provided herein are monoclonal antibodies specific to dengue virus as well as their antigen-binding fragments, and functional variants. Also disclosed are uses thereof for treating or diagnosing dengue virus infection.
1. An isolated antibody that specifically binds dengue virus serotype 2 envelope domain III, the antibody comprising a heavy chain CDR 1 including SEQ ID NO:2, a heavy chain CDR2 including SEQ ID NO:3, a heavy chain CDR3 including SEQ ID NO:4, a light chain CDR1 including SEQ ID NO:6, a light chain CDR2 including SEQ ID NO:7, and a light chain CDR3 including SEQ ID NO:8.
2. The isolated antibody of claim 1 , the antibody comprising a heavy chain variable region including SEQ ID NO:1 and a light chain variable region including SEQ ID NO:5.
3. The isolated antibody of claim 1 , wherein the antibody is an antigen-binding fragment.
4. The isolated antibody of claim 1 , wherein the antibody is humanized.
5. The isolated antibody of claim 1 , wherein the antibody is a chimeric antibody.
6. The isolated antibody of claim 1 , wherein the antibody is a single-chain antibody.
7. The isolated antibody of claim 3 , wherein the antigen-binding fragment is a F(ab′) 2 , Fab, or Fv.
8. A method for treating dengue virus serotype 2 infection, the method comprising administering to a subject in need thereof an effective amount of the antibody of claim 1 .
9. The method of claim 8 , wherein the antibody includes a heavy chain variable region including SEQ ID NO:1 and a light chain variable region including SEQ ID NO:5.
10. The method of claim 8 , wherein the antibody is humanized.
11. A method for detecting presence of a dengue virus antigen in a sample, the method comprising
providing a sample suspected of containing a dengue virus antigen,
contacting the sample with the antibody of claim 1 , and
determining whether the antibody binds to an antigen in the sample, wherein binding of the antibody to the antigen indicates presence of a dengue virus antigen in the sample, the antigen being dengue virus serotype 2 envelope domain III.
12. The method of claim 11 , wherein the antibody includes a heavy chain variable region including SEQ ID NO:1 and a light chain variable region including SEQ ID NO:5.
13. The method of claim 11 , wherein the antibody is humanized.
14. The method of claim 11 , wherein the sample is a tissue sample from a patient suspected of having dengue virus infection.
15. The method of claim 11 , wherein the sample is a serum sample from a patient suspected of having dengue virus infection.