Genes of an otitis media isolate of nontypeable
The invention relates to the polynucleotide sequence of a nontypeable stain of Haemophilus influenzae (NTHi) and polypeptides encoded by the polynucleotides and uses thereof. The invention also relates to NTHi genes which are upregulated during or in response to NTHi infection of the middle ear and/or the nasopharynx.
1. A method for detecting NTHi bacteria in a biological sample comprising
(a) contacting an isolated polynucleotide comprising the nucleotide sequence SEQ ID NO: 1193 with a biological sample under stringent hybridization conditions, wherein the hybridization conditions comprise washing with 0.015 M sodium chloride, 0.0015 M sodium citrate at 65-68° C., and
(b) detecting hybridization of the polynucleotide within the sample, wherein hybridization indicates the presence of NTHi bacteria in the biological sample.
2. The method of claim 1 wherein the biological sample is selected from the group consisting of serum, sputum, ear fluid, blood, urine, lymphatic fluid, and cerebrospinal fluid.
3. A viral vector comprising an isolated polynucleotide comprising the nucleotide sequence of SEQ ID NO: 1193.
4. A vector comprising an isolated polynucleotide comprising a nucleotide sequence of SEQ ID NO: 1196 that is operatively linked to a heterologous expression control sequence.
5. An isolated cell comprising the vector of claim 3 .