Method of detecting bioactive molecules in a fluid sample
A method of detecting the presence of bioactive molecules in a fluid sample, comprising contacting a solution of a microorganism selected from the group consisting of a monocellular algae and a cyanobacteria with the fluid sample so as to obtain a formulation having a microorganism concentration of 200 000-1×10 7 cells/mL of fluid sample; incubating the formulation for 10 to 120 minutes at a pH of 7 to 12 and a temperature between 18 and 35° C.; and measuring the fluorescence emitted by the formulation, whereby a fluorescence emitted in the sample that is lower than that in a control sample is an indication that the sample contains a bioactive molecule.
1. A method of detecting the presence of bioactive molecules in a fluid sample, comprising
activating a microorganism selected from the group consisting of a monocellular algae and a cyanobacteria under ambient light;
contacting a solution of the activated microorganism with the fluid sample so as to obtain a formulation having a microorganism concentration of 200,000-1×10 7 cells/mL of formulation;
incubating the formulation for 10 to 120 minutes at a pH of 7 to 12 and a temperature between 18 and 35° C. under ambient light followed by incubating the formulation for 5 to 120 minutes at a pH of 7 to 12 and a temperature between 18 and 35° C. under green light or darkness; and
measuring the fluorescence emitted by the formulation,
whereby a fluorescence emitted in the sample that is lower than that in a control sample is an indication that the sample contains a bioactive molecule.
2. The method of claim 1 , wherein the incubation under green light or darkness has a duration of about 5 to about 35 minutes.
3. The method of claim 1 , wherein the incubation under green light or darkness has a duration of about 15 minutes.
4. The method of claim 3 , wherein the incubation pH is between about 11 and 12.
5. The method of claim 4 , wherein the monocellular algae is Chlorella vulgaris.
6. The method of claim 5 , wherein the incubation temperature is about 35° C.
7. The method of claim 6 , wherein the activating of the Chlorella vulgaris is for at least 90 minutes.
8. The method of claim 7 , wherein the microorganism cellular concentration is about 2.5×10 6 cells/mL of formulation.
9. The method of claim 5 , wherein the bioactive molecule is selected from the group consisting of atrazine, diuron, glyphosate, chlorpyriphos, copper, lead, cadmium, mercury, cyanides, tetracycline, zinc, nickel and ammoniacal nitrogen.
10. The method of claim 5 , wherein the incubation under ambient light has a duration of about 10 to 15 minutes.
11. The method of claim 1 , wherein the monocellular algae is a Chlorophyceae.
12. The method of claim 11 , wherein the Chlorophyceae is a fresh water Chlorophyceae.
13. The method of claim 12 , wherein the microorganism cellular concentration is between about 2.5×10 6 and 5×10 6 cells/mL of formulation.
14. The method of claim 13 , wherein the bioactive molecule is selected from the group consisting of atrazine, diuron, glyphosate, chlorpyriphos, copper, lead, cadmium, mercury, cyanides, tetracycline, zinc, nickel and ammoniacal nitrogen.
15. The method of claim 12 , wherein the bioactive molecule is selected from the group consisting of atrazine, diuron, glyphosate, chlorpyriphos, copper, lead, cadmium, mercury, cyanides, tetracycline, zinc, nickel and ammoniacal nitrogen.
16. The method of claim 11 , wherein the monocellular algae is a marine Chlorophyceae.
17. The method of claim 16 , wherein the marine Chlorophyceae is Dunalliela tertiolecta.
18. The method of claim 17 , wherein the Dunalliela tertiolecta cellular concentration is between about 200,000 and 350,000 cells/mL of formulation.
19. The method of claim 17 , wherein the activating of the Dunalliela tertiolecta is for at least 45 minutes.
20. The method of claim 19 , wherein the bioactive molecule is selected from the group consisting of atrazine, diuron, glyphosate, malathion, chlorpyriphos, progesterone and dicrotophos.
21. The method of claim 1 , wherein the microorganism is Ankistrodesmus falcatus, Monoraphidium arcuatum, Scenedesmus quadricauda, Desmosdesmus subspicatus, Scenedesmus subpicatus, Scenedesmus obliquus, Pseudokirchneriella subspicata or Chlamydomonas reinhardtii.
22. The method of claim 1 , wherein the microorganism is a cyanobacteria.
23. The method of claim 22 , wherein the cyanobacteria is Anabaena sp.
24. The method of claim 23 , wherein the activating of the Anabaena sp. is for at least 60 minutes.
25. The method of claim 22 , wherein the cyanobacteria is Nostoc commune.
26. The method of claim 1 , wherein the microorganism is Phaeodactylum tricornutum, Nitzchia clostridium, Lasallia pustulata or Zostera caprocorni.
27. The method of claim 1 , wherein the monocellular algae is Chlorella vulgaris.
28. The method of claim 27 , wherein the formulation further comprises an additive, the additive being i) copper; ii) cadmium; or iii) a combination of copper and cadmium, and wherein the bioactive molecule is a metal ion.
29. The method of claim 28 , wherein the incubation temperature is about 35° C.
30. The method of claim 1 , wherein said detected fluorescence corresponds to wavelengths higher than 700 nm and said fluorescence is detected following illumination at a wavelength of 475,605 or 660 nm.
31. The method of claim 1 , wherein said monocellular algae or cyanobacteria is a stabilized monocellular algae or cyanobacteria.
32. The method of claim 1 , wherein the incubation under green light or darkness has a duration of about 10 minutes.
33. The method of claim 1 , wherein the incubation under ambient light has a duration of about 10 to 60 minutes.