Compositions and methods for 3-hydroxypropionate bio-production from biomass
Methods of obtaining mutant nucleic acid sequences that demonstrate elevated oxaloacetate α-decarboxylase activity are provided. Compositions, such as genetically modified microorganisms that comprise such mutant nucleic acid sequences, are described, as are methods to obtain the same.
1. A method for producing an acrylic acid-based consumer product, said method comprising the steps of:
i) combining a carbon source and a cell culture to produce 3-hydroxypropionic acid, wherein said cell culture comprises a genetically modified Escherichia coli ( E. coli ) microorganism comprising a heterologous nucleic acid encoding for a protein with malonyl-CoA reductase activity; and wherein said genetically modified E. coli microorganism further comprises reduction in activity of pyruvate formate-lyase;
ii) growing said cell culture in feed-batch, batch mode, or continuous batch mode production;
iii) converting said 3-hydroxypropionic acid to acrylic acid;
iv) polymerizing acrylic acid into a polymer of acrylate; and
v) processing said polymer of acrylate into a consumer product.
2. The method of claim 1 , wherein said microorganism is further modified for increased tolerance to 3-hydroxypropionic acid.
3. The method of claim 1 , wherein said genetically modified E. coli microorganism further comprises reduction in activity of lactate dehydrogenase, phosphate acetyltransferase, and pyruvate oxidase.
4. The method of claim 1 , wherein said genetically modified E. coli microorganism further comprises a modification in a malonyl-CoA reductase enzyme, wherein the malonyl-CoA reductase enzyme comprises a molecule with at least 90% sequence identity to SEQ ID NO: 11.
5. A consumer product produced according to the following steps:
i) combining a carbon source and a cell culture to produce 3-hydroxypropionic acid, wherein said cell culture comprises a genetically modified Escherichia coil microorganism comprising a heterologous nucleic acid encoding for a protein with malonyl-CoA reductase activity; and wherein said genetically modified E. coli microorganism further comprises reduction in activity of pyruvate formate-lyase;
ii) growing said cell culture in feed-batch, batch mode, or continuous batch mode production;
iii) converting said 3-hydroxypropionic acid to acrylic acid;
iv) polymerizing acrylic acid into a polymer of acrylate;and
v) processing said polymer of acrylate into a consumer product.
6. The method of claim 1 , wherein the polymer of acrylate is selected from the group consisting of methyl acrylate, butyl acrylate, ethyl acrylate, and polyacrylic acid.
7. The method of claim 1 , wherein the polymer of acrylate is butyl acrylate.
8. The method of claim 1 , wherein the polymer of acrylate is ethyl acrylate.
9. The method of claim 1 , wherein the consumer product is selected from the group consisting of: paints, coatings, plastics, adhesives, dispersives, textiles, or leather.
10. The method of claim 1 , wherein the cell culture has increased 3-HP production greater than about 40 g/L of biomass/cell culture.
11. The method of claim 1 , wherein the cell culture has increased 3-HP production greater than about 60 g/L of biomass/cell culture.
12. The method of claim 1 , wherein the cell culture has increased 3-HP production greater than about 85 g/L of biomass/cell culture.
13. The method of claim 1 , wherein said genetically modified E. coli microorganism further comprises reduction in activity of a lactate dehydrogenase.
14. The method of claim 1 , wherein said genetically modified E. coli microorganism further comprises reduction in activity of a phosphate acetyltransferase.
15. The method of claim 1 , wherein said genetically modified E. coli microorganism further comprises reduction in activity of a pyruvate oxidase.