IP Library Granted Patent US 8,663,969
Granted Patent B2
US 8,663,969 · App. 12/712,287 · Granted Mar 4, 2014

Hybrid mushroom strain J9277, its descendants, and related methods

Inventors: Richard W. Kerrigan (Kittanning, PA); Mark P. Wach (Allison Park, PA)
Assignee: Sylvan America, Inc.
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Quick Facts
Patent No.
US 8,663,969
App. No.
12/712,287
Granted
Mar 4, 2014
Kind
B2
Abstract

A mushroom culture of Agaricus bisporus produced by hybridization of a first strain and a second strain of Agaricus bisporus , wherein at least one of said first and second strains of Agaricus is a hybrid mushroom culture of Agaricus bisporus designated strain J9277. Diverse additional strains can be developed from J9277 by various means including somatic and tissue culture selection, basidiospore selection, and hybridization to other strains of Agaricus bisporus , and the resulting derivative strains can be screened for desirable commercial characteristics. One resultant class of the mushroom Agaricus bisporus (J. Lange) Imbach is the hybrid strain J10165. It exhibits an attractive appearance that includes a smooth, bright white cap, and is biologically incompatible with strains of the ‘U1’ lineage group. A method for improving facility hygiene and reducing disease incidence at any commercial Agaricus bisporus mushroom production facility is also provided.

Claims (7)

1. A method of producing mushroom culture of Agaricus bisporus comprising: hybridizing a first strain and a second strain of Agaricus bisporus , wherein at least one of said first and second strains of Agaricus is selected from the group consisting of a hybrid mushroom culture of Agaricus bisporus designated strain J9277, a representative culture of said hybrid strain J9277 having been deposited under ATCC Accession No. PTA-6692.

2. The method according to claim 1 , wherein said hybrid mushroom culture exhibits antagonism toward heterokaryon strains in the Horst U1/U3 lineage group.

3. The method according to claim 1 , wherein the mushroom culture is provided in mushroom products selected from the group consisting of inoculum, mushroom spawn, casing inoculant, and mushrooms.

4. The method according to claim 1 , wherein the mushroom culture is provided in derived cultures selected from the group consisting of homokaryons, heterokaryons, aneuploids, somatic subcultures, tissue explant cultures, protoplasts, live spores, germinating spores, inbred descendants and out crossed descendants, and transgenic lines.

5. The method according to claim 1 , further comprising: repeating the hybridization process of claim 1 for up to four generations of crosses.

6. The method according to claim 5 , wherein the mushroom culture is provided in mushroom products selected from the group consisting of inoculum, mushroom spawn, casing inoculant, and mushrooms.

7. The method according to claim 5 , wherein the mushroom culture is provided in derived cultures selected from the group consisting of homokaryons, heterokaryons, aneuploids, somatic subcultures, tissue explant cultures, protoplasts, live spores, germinating spores, inbred descendants and out crossed descendants, and transgenic lines.

Assignments (3)
CORRECTIVE ASSIGNMENT TO CORRECT THE ERRONEOUSLY FILED PATENT NUMBERS D804112 AND 9962249 PREVIOUSLY RECORDED ON REEL 059580 FRAME 0829. ASSIGNOR(S) HEREBY CONFIRMS THE NOTICE OF GRANT OF SECURITY INTEREST IN INTELLECTUAL PROPERTY. Recorded Aug 2, 2023
From: SYLVAN INC.; SYLVAN AMERICA, INC.; SYLVAN BIO, INC.
To: E.SUN COMMERCIAL BANK, LTD
Reel/Frame 064629/0864 →
NOTICE OF GRANT OF SECURITY INTEREST IN INTELLECTUAL PROPERTY Recorded Apr 4, 2022
From: SYLVAN INC.; SYLVAN AMERICA, INC.; SYLVAN BIO, INC.
To: E.SUN COMMERCIAL BANK, LTD
Reel/Frame 059580/0829 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Mar 3, 2010
From: KERRIGAN, RICHARD W; WACH, MARK P
To: SYLVAN AMERICA, INC.
Reel/Frame 024018/0651 →
Continuity (5)
Continuation In Part 12380199 · Feb 25, 2009
Continuation In Part 11433178 · May 12, 2006
Provisional Application 60680774 · May 13, 2005
Provisional Application 60682189 · May 17, 2005
Related Publication 20100154079A1 · Jun 17, 2010