Agent for recruitment of bone-marrow-derived pluripotent stem cell into peripheral circulation
The present invention for the first time demonstrated that: (1) bone marrow-derived pluripotent tissue stem cells can be induced in peripheral blood by intravenously administering tissue extract prepared from isolated skin pieces; (2) the substance in the isolated skin pieces, which is responsible for mobilizing bone marrow-derived pluripotent tissue stem cells to peripheral blood, is HMGB1; and (3) HMGB1 with the activity of mobilizing bone marrow-derived pluripotent stem cells to peripheral blood can be easily purified from cultured cells.
1. A method of mobilizing a bone marrow mesenchymal stem cell to peripheral blood from bone marrow, comprising the steps of administering to blood vessel or muscle of a living animal one or more substances selected from the group consisting of:
(a) an HMGB1 protein;
(b) a cell that secretes an HMGB1 protein;
(c) a vector inserted with a DNA encoding an HMGB1 protein;
(d) an HMGB2 protein;
(e) a cell that secretes an HMGB2 protein;
(f) a vector inserted with a DNA encoding HMGB2 protein;
(g) an HMGB3 protein;
(h) a cell that secretes an HMGB3 protein; and
(i) a vector inserted with a DNA encoding an HMGB3 protein; and mobilizing a bone marrow mesenchymal stem cell to peripheral blood from bone marrow.
2. The method according to claim 1 , wherein one or more substances selected from (a) to (i) are administered to blood vessel.
3. The method according to claim 2 , wherein one or more substances selected from (a) to (i) are administered via intravascular injection.
4. The method according to claim 3 , wherein one or more substances selected from (a) to (i) are administered via intraarterial injection or intravenous injection.
5. The method according to claim 1 , wherein one or more substances selected from (b) to (i) are administered.
6. The method according to claim 1 , wherein one or more substances from (d) to (i) are administered.
7. The method according to claim 1 , wherein the living animal is human.