BSL2vcvc-lg polypeptides
The present invention provides nucleic acids encoding B7-related factors that modulate the activation of immune or inflammatory response cells, such as T-cells. Also provided are expression vectors and fusion constructs comprising nucleic acids encoding B7-related polypeptides, including BSL1, BSL2, and BSL3. The present invention further provides isolated B7-related polypeptides, isolated fusion proteins comprising B7-related polypeptides, and antibodies that are specifically reactive with B7-related polypeptides, or portions thereof. In addition, the present invention provides assays utilizing B7-related nucleic acids, polypeptides, or peptides. The present invention further provides compositions of B7-related nucleic acids, polypeptides, fusion proteins, or antibodies that are useful for the immunomodulation of a human or animal subject.
1. An isolated fusion protein consisting of a first polypeptide corresponding to amino acids 29 to 534 of SEQ ID NO:7 fused to a second polypeptide corresponding to the Fc domain of an immunoglobulin.
2. The isolated fusion protein of claim 1 , wherein said first polypeptide is encoded by nucleotides 205 to 1722 of SEQ ID NO:6.
3. An isolated fusion protein selected from the group consisting of:
(a) an isolated fusion protein comprising amino acids 1 to 698 of SEQ ID NO:9;
(b) an isolated fusion protein comprising amino acids 2 to 698 of SEQ ID NO:9; and
(c) an isolated fusion protein comprising amino acids 29 to 698 of SEQ ID NO:9.
4. The isolated fusion protein of claim 3 , wherein said fusion protein of (a) comprises a polypeptide encoded by nucleotides 1 to 2094 of SEQ ID NO:8.
5. The isolated fusion protein of claim 3 wherein said fusion protein of (b) comprises a polypeptide encoded by nucleotides 4 to 2094 of SEQ ID NO:8.
6. The isolated fusion protein of claim 3 , wherein said fusion protein of (c) comprises a polypeptide encoded by nucleotides 85 to 2094 of SEQ ID NO:8.
7. An isolated polypeptide made by a method comprising:
(a) culturing a recombinant host cell comprising a vector that comprises a polynucleotide encoding the fusion protein selected from the group consisting of the fusion protein of claim 1 , the fusion protein of claim 2 , and the fusion protein of claim 3 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.
8. An isolated fusion protein selected from the group consisting of:
(a) an isolated fusion protein comprising amino acids 29 to 465 of SEQ ID NO:7 as encoded by the polynucleotide contained in plasmid BSL2-4616811 of ATCC Deposit No. PTA-1993 fused to a second polypeptide corresponding to the Fc domain of an immunoglobulin; and
(b) an isolated fusion protein encoded by the BSL2vcvc-Ig cDNA contained in ATCC Deposit No. PTA-4056.
9. An isolated fusion protein consisting of the extracellular domain of SEQ ID NO:7 fused to a heterologous polypeptide corresponding to the Fc domain of an immunoglobulin, wherein said extracellular domain is amino acids 1 to 465 of SEQ ID NO:7.
10. An isolated fusion protein consisting of the mature extracellular domain of SEQ ID NO:7 fused to a heterologous polypeptide corresponding to the Fc domain of an immunoglobulin.
11. The isolated fusion protein according to claim 10 wherein said mature extracellular domain is amino acids 29 to 465 of SEQ ID NO:7.
12. An isolated fusion protein consisting of amino acids 2 to 465 of SEQ ID NO:7 fused to a heterologous polypeptide corresponding to the Fc domain of an immunoglobulin.
13. The isolated fusion protein according to claim 1 , 3 , 8 , 9 , 10 or 12 , wherein said immunoglobulin is IgG.
14. An isolated polypeptide made by a method comprising:
(a) culturing a recombinant host cell comprising a vector that comprises a polynucleotide encoding the fusion protein selected from the group consisting of the fusion protein of claim 9 , the fusion protein of claim 10 , and the fusion protein of claim 11 under conditions such that said polypeptide is expressed; and
(b) recovering said polypeptide.