IP Library › Granted Patent US 8,679,798
Granted Patent B2
US 8,679,798 · App. 12/202,479 · Granted Mar 25, 2014

Method for producing an L-amino acid using a bacterium of the

Inventors: Tatyana Abramovna Yampolskaya (Moscow, RU); Natalia Viktorovna Stoynova (Moscow, RU); Natalia Sergeevna Eremina (Moscow, RU); Irina Borisovna Altman (Moscow, RU); Leonid Romanovich Ptitsyn (Moscow, RU)
Assignee: Ajinomoto Co., Inc.
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Quick Facts
Patent No.
US 8,679,798
App. No.
12/202,479
Granted
Mar 25, 2014
Kind
B2
Abstract

The present invention provides a method for producing an L-amino acid from ethanol using a bacterium of the Enterobacteriaceae family, wherein the bacterium has been modified to enhance the expression of the ydbK gene.

Claims (7)

1. A method for producing L-threonine and/or L-valine comprising:

cultivating an Escherichia coli which produces L-threonine and/or L-valine in a medium containing ethanol as the sole carbon source, and

collecting said L-threonine and/or L-valine from the medium,

wherein said Escherichia coli has been modified to enhance the expression of the ydbK gene.

2. The method according to claim 1 , wherein said expression is enhanced by modifying an expression control sequence of the ydbK gene or by increasing the copy number of the ydbK gene.

3. The method according to claim 1 , wherein said ydbK gene encodes a protein having a homology of not less than 95% with respect to the amino acid sequence shown in SEQ ID NO. 2.

4. The method according to claim 1 , wherein said ydbK gene encodes a protein comprising the amino acid sequence shown in SEQ ID NO. 2.

Assignments (1)
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Dec 10, 2008
From: YAMPOLSKAYA, TATYANA ABRAMOVNA; STOYNOVA, NATALIA VIKTOROVNA; EREMINA, NATALIA SERGEEVNA; ALTMAN, IRINA BORISOVNA; PTITSYN, LEONID ROMANOVICH
To: AJINOMOTO CO., INC.
Reel/Frame 021956/0662 →
Priority Claims (1)
RU 2007147434 · Dec 21, 2007 · national
Continuity (1)
Related Publication 20090162908A1 · Jun 25, 2009