Simian E adenoviruses SAdV-39, -25.2, -26, -30, -37, and -38
A recombinant vector comprises simian adenovirus SAdV-39, -25.2, -26, -30, -37, and -38 sequences and a heterologous gene under the control of regulatory sequences. A cell line which expresses simian adenovirus SAdV-39, -25.2, -26, -30, -37, and -383 gene(s) is also disclosed. Methods of using the vectors and cell lines are provided.
1. An adenovirus having a capsid comprising a hexon protein of SAdV-39 with the amino acids 1 to 940 of SEQ ID NO: 11; and a fiber protein of said SAdV-39 with the amino acids 1 to 489 of SEQ ID NO: 22;
said capsid encapsidating a heterologous nucleic acid comprising a gene operably linked to expression control sequences which direct transcription, translation, and/or expression thereof in a host cell.
2. The adenovirus according to claim 1 , further comprising a 5′ and a 3′ adenovirus cis-element necessary for replication and encapsidation.
3. The adenovirus according to claim 1 , wherein said adenovirus lacks all or a part of the E1 gene.
4. The adenovirus according to claim 3 , wherein said adenovirus is replication-defective.
5. The adenovirus according to claim 1 , wherein said capsid is a hybrid capsid.
6. The adenovirus according to claim 5 , wherein the hybrid capsid comprises at least one capsid protein from one or more adenoviruses selected from SAdV-30, SAdV-25.2, SAdV-37, SAdV-38, and SAdV-26.
7. The adenovirus according to claim 1 , wherein the capsid further comprises a penton protein of an adenovirus selected from SAdV-39, SAdV-30, SAdV-25.2, SAdV-37, SAdV-38 and SAdV-26.
8. The adenovirus according to claim 1 , wherein said adenovirus is a pseudotyped adenovirus.
9. The adenovirus according to claim 1 , wherein the recombinant adenovirus comprises one or more adenovirus genes.
10. A composition comprising the adenovirus according to claim 1 , further comprising one or more simian adenovirus proteins selected from the group consisting of:
E1a, SEQ ID NO: 30;
E1b, small T/19K, SEQ ID NO: 24;
E1b, large T/55K, SEQ ID NO: 2;
IX, SEQ ID NO: 3;
52/55D, SEQ ID NO: 4;
IIIa, SEQ ID NO: 5;
Penton, SEQ ID NO: 6;
VII, SEQ ID NO: 7;
V, SEQ ID NO: 8;
pX, SEQ ID NO: 9;
VI, SEQ ID NO: 10;
Endoprotease, SEQ ID NO: 12;
100 kD, SEQ ID NO: 13;
33 kD, SEQ ID NO: 32;
22 kD, SEQ ID NO: 26;
VIII, SEQ ID NO: 14;
E3/12.5 K, SEQ ID NO: 15;
CR1-alpha, SEQ ID NO: 27;
gp19K, SEQ ID NO: 16;
CR1-beta, SEQ ID NO: 17;
CR1-gamma, SEQ ID NO: 18;
CR1-delta, SEQ ID NO: 19;
RID-alpha, SEQ ID NO: 20;
RID-beta, SEQ ID NO: 21; and
E3/14.7K, SEQ ID NO: 28.
11. A composition comprising the adenovirus according to claim 1 in a pharmaceutically acceptable carrier.