Monocot seed product comprising a human blood protein
The invention is directed to blood proteins produced in monocot seeds and isolated therefrom for use in therapeutic compositions, as well as to methods of making these isolated blood proteins and to therapeutic compositions comprising them.
1. A method of producing a recombinant human blood protein in monocot plant seeds, comprising the steps of:
(a) transforming a monocot plant cell with a chimeric gene comprising
(i) a promoter from the gene of a maturation-specific monocot plant storage protein operably linked to a first DNA sequence encoding a monocot plant seed-specific signal sequence capable of targeting a polypeptide linked thereto to a monocot plant seed endosperm cell, wherein the promoter operably linked to the sequence encoding the signal sequence comprises SEQ ID NO: 7; and
(ii) a second DNA sequence encoding the human blood protein, linked in translation frame with the first DNA sequence, wherein the first DNA sequence and the second DNA sequence together encode a fusion protein comprising an N-terminal signal sequence and the human blood protein;
(b) growing a monocot plant from the transformed monocot plant cell for a time sufficient to produce seeds containing the human blood protein; and
(c) harvesting the seeds from the plant, wherein the human blood protein constitutes at least about 3.0% of the total soluble protein in the harvested seeds.
2. The method of claim 1 , wherein the human blood protein is selected from the group consisting of hemoglobin, alpha-1-antitrypsin, fibrinogen, human serum albumin (HSA), thrombin, antithrombin Ill, an antibody, a growth factor and a blood coagulation factor.
3. The method of claim 1 , wherein the human blood protein constitutes at least about 5.0% of the total soluble protein in the harvested seeds.
4. The method of claim 1 , wherein the human blood protein constitutes at least about 10.0% of the total soluble protein in the harvested seeds.
5. The method of claim 1 , further comprising purifying the human blood protein from the harvested seeds.
6. The method of claim 1 , wherein the human blood protein produced in the method comprises one or more plant glycosyl groups.
7. A composition comprising a purified recombinant human blood protein obtained by the method of claim 1 , wherein the human blood protein comprises one or more plant glycosyl groups; and wherein said composition comprises SEQ ID NO: 7.
8. The composition of claim 7 , wherein the human blood protein is selected from the group consisting of hemoglobin, alpha-1-antitrypsin, fibrinogen, human serum albumin (HSA), thrombin, antithrombin III, an antibody, a growth factor and a blood coagulation factor.
9. A monocot plant seed product selected from the group consisting of whole seed, flour, extract, malt, and a partially purified protein fraction, prepared from the harvested seeds obtained by the method of claim 1 , wherein the human blood protein constitutes at least about 3.0% of the total soluble protein in the seed product; and wherein the seed product comprises SEQ ID NO: 7.
10. The seed product of claim 9 , wherein the human blood protein constitutes at least about 5.0% of the total soluble protein in the seed product.
11. The seed product of claim 9 , wherein the human blood protein constitutes at least about 10.0% of the total soluble protein in the seed product.
12. The seed product of claim 9 , wherein the human blood protein is selected from the group consisting of hemoglobin, alpha-1-antitrypsin, fibrinogen, human serum albumin (HSA), thrombin, antithrombin III, an antibody, a growth factor and a blood coagulation factor.
13. A composition comprising:
a purified recombinant human blood protein obtained by the method of claim 1 , wherein the human blood protein comprises one or more plant glycosyl groups; and
at least one pharmaceutically acceptable excipient or nutrient from a source other than the monocot plant, wherein the excipient or nutrient is selected from the group consisting of salts, saccharides, vitamins, minerals, amino acids, peptides and proteins other than the human blood protein;
wherein said composition comprises SEQ ID NO: 7.
14. The composition of claim 13 , wherein the human blood protein is selected from the group consisting of hemoglobin, alpha-1-antitrypsin, fibrinogen, human serum albumin (HSA), thrombin, antithrombin Ill, an antibody, and a blood coagulation factor.
15. The composition of claim 13 , wherein the composition is formulated for parenteral, enteric, inhalation, intranasal or topical delivery.
16. The composition of claim 13 , wherein the composition is formulated for enteric delivery.