Mutant RB69 DNA polymerase
Provided herein are mutant DNA-dependent polymerases which are derived from, or otherwise related to, wild type RB69 DNA polymerase. These mutant polymerases are capable of selectively binding labeled nucleotides. These mutant polymerases are also capable of incorporating a variety of naturally occurring and modified nucleotides, including, for example, terminator nucleotides.
1. An isolated mutant DNA polymerase comprising the amino acid sequence of SEQ ID NO:2.
2. The isolated mutant DNA polymerase of claim 1 , which binds a labeled nucleotide.
3. The isolated mutant DNA polymerase of claim 1 , which incorporates a reversible terminator nucleotide having an azidomethyl blocking group on the 3′ sugar position.
4. The isolated mutant DNA polymerase of claim 1 further comprising a reporter moiety.
5. The isolated mutant DNA polymerase of claim 4 , wherein the reporter moiety is an energy transfer donor moiety.
6. The isolated mutant DNA polymerase of claim 5 , wherein the energy transfer donor moiety is a fluorescent dye or a nanoparticle.
7. A system comprising a mutant DNA polymerase of claim 1 bound to a DNA template and a primer, and a nucleotide transiently-bound to the mutant DNA polymerase.