Agents and methods for tissue repair and regeneration
View Patent ↗Methods of treating solid organ injuries using compounds that enhance Wnt signalling are described.
1. A method for enhancing tissue repair and regeneration following injury in a kidney or pancreas, the method comprising administering a therapeutically effective amount of a composition comprising an agent that enhances canonical Wnt signaling, wherein the agent is selected from the group consisting of human Dikkopf 2 (Dkk2), mouse DKK2, an active fragment of mouse Dkk2 comprising SEQ ID NO: 91, and an active fragment of human Dkk2 comprising SEQ ID NO:90, and wherein the tissue repair and regeneration following injury in a kidney or pancreas is enhanced.
2. The method of claim 1 , wherein the injury is an injury in which activated macrophages play a role in recovery.
3. The method of claim 2 , wherein the injury is an ischemic injury.
4. The method of claim 1 , wherein the composition enhances regeneration of epithelial cells.
5. The method of claim 1 , wherein the composition enhances regeneration of vasculature in the kidney.
6. The method of claim 1 , wherein the composition is administered systemically or by infusion through the vasculature.
7. The method of claim 1 , wherein the composition enhances LRP5/6 or Fzd 4/7/8 activity in epithelial cells.
8. The method of claim 1 , wherein the human DKK2 comprises SEQ ID NO:92.
9. The method of claim 1 , wherein the mouse DKK2 comprises SEQ ID NO:93.
10. The method of claim 2 , wherein the injury is an inflammatory injury.
11. The method of claim 2 , wherein the injury is a toxic injury.
12. The method of claim 2 , wherein the injury is an infectious injury.
13. The method of claim 2 , wherein the injury is an autoimmune injury.
14. The method of claim 1 , wherein the composition enhances regeneration of vasculature in the pancreas.
15. A method for enhancing tissue repair and regeneration following injury in a kidney, the method comprising administering a therapeutically effective amount of a composition comprising an active fragment of human Dkk2 comprising SEQ ID NO:90, and wherein the tissue repair and regeneration following injury in a kidney is enhanced.
16. A method for enhancing tissue repair and regeneration following injury in a pancreas, the method comprising administering a therapeutically effective amount of a composition comprising an active fragment of human Dkk2 comprising SEQ ID NO:90, and wherein the tissue repair and regeneration following injury in a pancreas is enhanced.