Systems and methods for cryopreservation of cells
A fluid sample vessel includes inlet and vent tube fittings formed at one end of a container with an opposite open end closed by a needle septum. A support cap is removably engaged to the container to support the container and protect terminal ends of inlet and vent tubular branches coupled to the fittings. The support cap includes a pair of opposite legs with outwardly directed tabs for mounting within a centrifuge while supporting the cryopreservation container.
1. A device for receiving a liquid sample obtained from a separate container, comprising:
a container for receiving the liquid sample, said container formed by a one-piece elongated body, said body defining a hollow interior from an open end and being closed at the opposite end by an inlet tube fitting and a vent tube fitting opening to said hollow interior, said one-piece body further defining a wall extending into said hollow interior and disposed between said inlet and vent tube fittings;
an adaptor body mounted to said tube fittings, said adaptor body having an inlet tubular branch and a vent tubular branch, said vent tubular branch including a filter element disposed therein; and
a needle septum closing said open end.
2. The device of claim 1 , wherein said inlet tubular branch includes a flared end adapted to receive an adapter for coupling said branch to a separate tube associated with a liquid container.
3. The device of claim 1 , further comprising a gas-permeable sterile micro-filter disposed within said vent tube fitting.
4. The device of claim 3 , wherein said gas-permeable sterile micro-filter is configured to be generally liquid impermeable.
5. The device of claim 1 , further comprising a removable septum cover operable to protect said needle septum against exposure when subjected to cryopreservation conditions.
6. The device of claim 1 , wherein the container is formed of a plastic suitable for storing a blood sample.
7. The device of claim 1 , wherein the container is formed of a plastic suitable for storing the liquid sample at cryogenic temperatures.
8. The device of claim 1 , also comprising a length of heat-sealable tubing coupled to said inlet tube fitting.
9. The device of claim 1 , also comprising a cryoprotectant received in the container.
10. The device of claim 9 , also comprising cells received in the container.
11. A device for receiving a liquid sample obtained from a separate container, comprising:
a container for receiving the liquid sample, said container formed by a one-piece elongated body, said body defining a hollow interior from an open end and being closed at the opposite end by an inlet tube fitting and a vent tube fitting opening to said hollow interior, said one-piece body further defining a wall extending into said hollow interior and disposed between said inlet and vent tube fittings;
a support cap having a lower portion removably engagable to said container and an upper portion extending beyond said inlet and vent tube fittings at said opposite end of said container when the support cap is engaged to the container; and
a needle septum closing said open end.
12. The device of claim 11 , wherein:
said lower portion of said support cap includes at least two resiliently deflectable elongated prongs, each having an inwardly directed tab configured to be removably received within a corresponding one of said recesses.
13. The device of claim 11 , further comprising a gas-permeable sterile micro-filter disposed within said vent tube fitting.
14. The device of claim 13 , wherein said gas-permeable sterile micro-filter is configured to be generally liquid impermeable.
15. The device of claim 11 , further comprising a removable septum cover operable to protect said needle septum against exposure when subjected to cryopreservation conditions.
16. The device of claim 11 , wherein the container is formed of a plastic suitable for storing a blood sample.
17. The device of claim 11 , wherein the container is formed of a plastic suitable for storing the liquid sample at cryogenic temperatures.
18. The device of claim 11 , also comprising a length of heat-sealable tubing coupled to said inlet tube fitting.
19. The device of claim 11 , also comprising a cryoprotectant received in the container.
20. The device of claim 19 , also comprising cells received in the container.