IP Library Granted Patent US 8,728,550
Granted Patent B2
US 8,728,550 · App. 13/102,964 · Granted May 20, 2014

Animal meal that includes fungal biomass

Inventors: Suresh M. Menon (San Diego, CA); Arul Victor Suresh (San Diego, CA); Samantha S. Orchard (San Diego, CA); Kashinatham Alisala (San Diego, CA); Jagadish C. Sircar (San Diego, CA); Sara Ingegard Hash (San Marcos, CA)
Assignee: Menon Renewable Resources, Inc.
A23K1/1813C12R1/645A23K1/164A23K1/1826A23K1/1631A23K1/1634A23K1/1853A23K1/003C12N1/14A01N63/04A23L1/3008A23V2002/00Y10S426/805Y10S426/807
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Quick Facts
Patent No.
US 8,728,550
App. No.
13/102,964
Granted
May 20, 2014
Kind
B2
Abstract

Disclosed herein are compositions comprising an isolated cellulose degrading fungus. Also disclosed are culture compositions and bioreactor compositions comprising the cellulose degrading fungus. Further described herein are filtration and extraction devices comprising the cellulose degrading fungus. Still further disclosed are bioprocessing facilities for and methods for producing co-products resulting from one or more bioprocesses of the cellulose degrading fungus.

Claims (44)

1. An admixture comprising fungal biomass and whey, wherein said fungal biomass comprises a fungus selected from the group consisting of: a fungus having a 5.8S ribosomal RNA gene sequence with at least 98% nucleotide sequencing identity to the nucleic acid of SEQ ID NO: 1; a fungus having an ITS1 sequence with at least 98% sequence identity to SEQ ID NO: 2; a fungus having an ITS2 sequence with at least 98% sequence identity to SEQ ID NO: 3; a fungus having a 28S ribosomal RNA gene sequence with at least 98% sequence identity to SEQ ID NO: 4 , Penicillium menonorum and a species being the same as NRRL deposit Accession No: 50410.

2. The admixture of claim 1 , wherein said fungal biomass has been substantially depleted of a lipid product.

3. The admixture of claim 1 , said admixture being suitable for animal consumption.

4. The admixture of claim 1 , wherein said fungal biomass and whey are in a dry fungal biomass/whey ratio ranging from about 1:100 to about 100:1 (vol/vol).

5. The admixture of claim 1 wherein the fungal biomass is a product of aerobic fermentation of a feedstock comprising biological matter.

6. The admixture of claim 5 wherein the biological matter comprises at least one of: simple sugars, cane juice or its condensates or its dry solid evaporate, whey, grains, stover, forage, grasses, oilseed crops, nut shells, nut hulls, fruit pomace, plant waste, algae, wood, wood byproducts, bark, paper, paper products, animal manure, food waste, yard waste, almond hulls, grape pomace, agriculture waste, sawdust.

7. The admixture of claim 5 wherein the biological matter comprises at least one of: a cellulosic carbon source or a lysate or a hydrolysate thereof, a hemicellulosic carbon source or a lysate or a hydrolysate thereof, and a lignocellulosic carbon source or a lysate or a hydrolysate thereof.

8. The admixture of claim 1 wherein the fungal biomass comprises greater than 25% cell dry weight (cdw) crude protein.

9. The admixture of claim 1 wherein the fungal biomass comprises between 1% and 90% of the admixture by weight.

10. The admixture of claim 1 wherein the fungal biomass comprises at least 10% (cdw) lipid comprising triacylglycerol (TAG).

11. The admixture of claim 1 wherein the fungal biomass comprises mycelial residue following extraction of lipids.

12. The admixture of claim 11 wherein the lipids comprise at least one of: TAG, wax esters, mono-unsaturated hydrocarbon chains, di-unsaturated hydrocarbon chains, and any molecular forms having a straight-chain saturated hydrocarbon portion.

13. The admixture of claim 1 wherein the fungal biomass comprises biomass harvested at a particular time to optimize production of a non-lipid portion of the biomass, the particular time being either a time that an available nitrogen ingredient is exhausted, or a time when log-phase growth terminates.

14. The admixture of claim 1 , wherein said fungus has a 5.8S ribosomal RNA gene sequence with at least 98% nucleotide sequencing identity to the nucleic acid of SEQ ID NO: 1.

15. The admixture of claim 1 , wherein said fungus has a 5.8S ribosomal RNA gene sequence with at least 99% nucleotide sequencing identity to the nucleic acid of SEQ ID NO: 1.

16. The admixture of claim 1 , wherein said fungus has a 5.8S ribosomal RNA gene sequence of SEQ ID NO: 1.

17. The admixture of claim 1 , wherein said fungus has an ITS1 sequence with at least 98% sequence identity to SEQ ID NO: 2.

18. The admixture of claim 1 , wherein said fungus has an ITS1 sequence with at least 99% sequence identity to SEQ ID NO: 2.

19. The admixture of claim 1 , wherein said fungus has an ITS1 sequence of SEQ ID NO: 2.

20. The admixture of claim 1 , wherein said fungus has an ITS2 sequence with at least 98% sequence identity to SEQ ID NO: 3.

21. The admixture of claim 1 , wherein said fungus has an ITS2 sequence with at least 99% sequence identity to SEQ ID NO: 3.

22. The admixture of claim 1 , wherein said fungus has an ITS2 sequence of SEQ ID NO: 3.

23. The admixture of claim 1 , wherein said fungus has a 28S ribosomal RNA gene sequence with at least 98% sequence identity to SEQ ID NO: 4.

24. The admixture of claim 1 , wherein said fungus has a 28S ribosomal RNA gene sequence with at least 99% sequence identity to SEQ ID NO: 4.

25. The admixture of claim 1 , wherein said fungus has a 28S ribosomal RNA gene sequence of SEQ ID NO: 4.

26. The admixture of claim 1 , wherein said fungus is Penicillium menonorum.

27. The admixture of claim 1 , wherein said fungus is a species being the same as NRRL deposit Accession No: 50410.

28. The admixture of claim 1 , wherein said fungal biomass and whey are in a dry fungal biomass/whey ratio ranging from about 1:1 to about 20:1 (vol/vol).

29. The admixture of claim 1 , wherein said fungal biomass and whey are in a dry fungal biomass/whey ratio ranging from about 5:1 to about 20:1 (vol/vol).

30. The admixture of claim 1 wherein the fungal biomass comprises about 25% to 75% (cdw) crude protein.

31. The admixture of claim 1 wherein the fungal biomass comprises between 80% and 95% of the admixture by weight.

32. The admixture of claim 1 , wherein the admixture is in the form of a pellet.

33. The admixture of claim 1 , wherein the admixture is in the form of a slurry.

34. The admixture of claim 1 , wherein the fungal biomass is produced by fermentation of a feedstock with said fungus in a liquid culture, removing a fungal biomass from the liquid culture, and drying the fungal biomass.

35. The admixture of claim 34 , wherein the admixture is produced by combining the dried fungal biomass with whey.

36. The admixture of claim 35 , wherein feedstock comprises whey.

37. The admixture of claim 1 , wherein the admixture is a product of fermentation of a feedstock comprising whey with said fungus.

38. The admixture of claim 1 , wherein said fungus is Penicillium menonorum , wherein the fungal biomass is dried prior to combining with whey, wherein the fungal biomass comprises about 25% to 75% (cdw) crude protein, and wherein the admixture comprises between 80% and 95% fungal biomass by weight.

39. The admixture of claim 38 , wherein said fungal biomass has been substantially depleted of a lipid product.

40. The admixture of claim 1 , wherein said fungal biomass comprises a fatty acid.

41. The admixture of claim 40 , wherein the fatty acid is selected from the group consisting of myristic acid, pentadecylic acid, palmitic acid, margaric acid, stearic acid, oleic acid, linoleic acid, linolenic acid, arachic acid, behenic acid, lignoceric acid and combinations thereof.

42. The admixture of claim 41 , wherein the fatty acid is selected from the group consisting of palmitic acid, oleic acid, and linoleic acid.

43. The admixture of claim 41 , wherein the fatty acid is linolenic acid.

44. The admixture of claim 43 , wherein the fatty acid is present as TAG, and at least 3.0% of the TAG is linolenic acid.

Assignments (8)
RELEASE OF SECURITY INTEREST Recorded Dec 2, 2019
From: BIG CATCH/MRP CAYMAN, LTD
To: MENON RENEWABLE PRODUCTS, INC.
Reel/Frame 051158/0525 →
ASSUMPTION Recorded Sep 11, 2017
From: BUMBLE BEE FOODS, LLC
To: BIG CATCH/MRP CAYMAN LTD.
Reel/Frame 043811/0740 →
SECURITY INTEREST Recorded Jun 13, 2016
From: MENON RENEWABLE PRODUCTS, INC.
To: BUMBLE BEE FOODS, LLC
Reel/Frame 038980/0511 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Apr 21, 2016
From: HASH, SARA INGEGARD
To: MENON RENEWABLE PRODUCTS, INC.
Reel/Frame 038346/0708 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Nov 19, 2014
From: MENON INTERNATIONAL, INC.
To: MENON RENEWABLE PRODUCTS, INC.
Reel/Frame 034214/0101 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Jan 24, 2014
From: HASH, SARA INGEGARD
To: MENON RENEWABLE RESOURCES, INC.
Reel/Frame 032043/0571 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Oct 14, 2013
From: MENON & ASSOCIATES, INC.
To: MENON INTERNATIONAL, INC.
Reel/Frame 031401/0972 →
ASSIGNMENT OF ASSIGNOR'S INTEREST Recorded Aug 1, 2012
From: MENON, SURESH M.; SURESH, ARUL VICTOR; ORCHARD, SAMANTHA S.; ALISALA, KASHINATHAM; SIRCAR, JAGADISH C.
To: MENON & ASSOCIATES, INC.
Reel/Frame 028698/0403 →
Continuity (5)
Provisional Application 61344011 · May 7, 2010
Provisional Application 61372828 · Aug 11, 2010
Provisional Application 61411860 · Nov 9, 2010
Provisional Application 61475176 · Apr 13, 2011
Related Publication 20120052150A1 · Mar 1, 2012