Method for prediction of the progression risk of tumors
The present invention concerns a method for predicting the potential for aggressive growth and/or the risk to progress to high grade cancer for tumors in cell based detection procedures. In one aspect the invention concerns the detection of overexpression of cyclin-dependent kinase inhibitor gene products as a tool for predicting the progression risk and/or potential for aggressive growth of tumors. In a second aspect the invention concerns predicting the progression risk and/or potential for aggressive growth in tumors on the basis of the simultaneous co-detection of the presence of overexpression of cyclin-dependent kinase inhibitor gene products together with the expression of markers for active cell proliferation. Further the invention concerns preparations of probes for diagnosis namely for predicting the progression risk and/or the potential for aggressive growth of tumors.
1. A method for predicting a potential for tumors of the urinary system for aggressive growth and/or a risk to progress to high grade cancer from low grade cancer, comprising:
obtaining a tumor sample from the urinary system of a subject;
measuring by a cell based detection procedure whether at least one single cell of the sample simultaneously overexpresses p16INK4a and expresses Ki67; and
determining that the tumors have a potential for aggressive growth and/or a risk to progress to high grade cancer when there is at least one single cell in the sample having the simultaneous presence of overexpression of p16INK4a and the expression of Ki67.
2. The method of claim 1 , wherein the cell based detection procedure is immunohistochemistry or immunocytochemistry.
3. The method of claim 1 , wherein the tumor is bladder cancer.
4. The method of claim 1 , said measuring uses antibodies against p16INK4a and Ki67.
5. The method of claim 1 , wherein the sample is a tissue sample or a cell sample.